Nucleus-encoded, plastid-targeted glyceraldehyde-3-phosphate dehydrogenase (GAPDH) indicates a single origin for chromalveolate plastids

被引:142
作者
Harper, JT [1 ]
Keeling, PJ [1 ]
机构
[1] Univ British Columbia, Dept Bot, Canadian Inst Adv Res, Vancouver, BC, Canada
关键词
alveolates; apicomplexans; dinollagellates; GAPDH; haptophytes; heterokonts;
D O I
10.1093/molbev/msg195
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plastids (the photosynthetic organelles of plants and algae) originated through endosymbiosis between a cyanobacterium and a eukaryote and subsequently spread to other eukaryotes by secondary endosymbioses between two eukaryotes. Mounting evidence favors a single origin for plastids of apicomplexans, cryptophytes, dinoflagellates, haptophytes, and heterokonts, (together with their nonphotosynthetic relatives, termed chromalveolates), but so far, no single molecular marker has been described that supports this common origin. One piece of evidence comes from plastid-targeted glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which originated by a gene duplication of the cytosolic form. However, no plastid GAPDH has been characterized from haptophytes, leaving an important piece of the puzzle missing. We have sequenced genes encoding cytosolic, mitochondrion-targeted, and plastid-targeted GAPDH proteins from a number of haptophytes and heterokonts and found haptophyte homologs that branch within a strongly supported clade of chromalveolate plastid-targeted genes, being more closely related to an apicomplexan homolog than was expected. The evolution of plastid-targeted GAPDH supports red algal ancestry of apicomplexan plastids and raises a number of questions about the importance of plastid loss and the possibility of cryptic plastids in nonphotosynthetic lineages such as ciliates.
引用
收藏
页码:1730 / 1735
页数:6
相关论文
共 41 条
[1]   A cyanobacterial gene in nonphotosynthetic protists - An early chloroplast acquisition in eukaryotes? [J].
Andersson, JO ;
Roger, AJ .
CURRENT BIOLOGY, 2002, 12 (02) :115-119
[2]   Recycled plastids: a 'green movement' in eukaryotic evolution [J].
Archibald, JM ;
Keeling, PJ .
TRENDS IN GENETICS, 2002, 18 (11) :577-584
[3]   A kingdom-level phylogeny of eukaryotes based on combined protein data [J].
Baldauf, SL ;
Roger, AJ ;
Wenk-Siefert, I ;
Doolittle, WF .
SCIENCE, 2000, 290 (5493) :972-977
[4]   Phylogenetic relationships among algae based on complete large-subunit rRNA sequences [J].
Ben Ali, A ;
De Baere, R ;
Van der Auwera, G ;
De Wachter, R ;
Van de Peer, Y .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :737-749
[5]   The non-photosynthetic plastid in malarial parasites and other apicomplexans is derived from outside the green plastid lineage [J].
Blanchard, JL ;
Hicks, JS .
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1999, 46 (04) :367-375
[6]   Weighted neighbor joining: A likelihood-based approach to distance-based phylogeny reconstruction [J].
Bruno, WJ ;
Socci, ND ;
Halpern, AL .
MOLECULAR BIOLOGY AND EVOLUTION, 2000, 17 (01) :189-197
[7]   A revised six-kingdom system of life [J].
Cavalier-Smith, T .
BIOLOGICAL REVIEWS, 1998, 73 (03) :203-266
[8]   Principles of protein and lipid targeting in secondary symbiogenesis: Euglenoid, dinoflagellate, and sporozoan plastid origins and the eukaryote family tree [J].
Cavalier-Smith, T .
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1999, 46 (04) :347-366
[9]   Genomic reduction and evolution of novel genetic membranes and protein-targeting machinery in eukaryote-eukaryote chimaeras (meta-algae) [J].
Cavalier-Smith, T .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2003, 358 (1429) :109-133
[10]   DETERMINANTS OF COENZYME SPECIFICITY IN GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - ROLE OF THE ACIDIC RESIDUE IN THE FINGERPRINT REGION OF THE NUCLEOTIDE-BINDING FOLD [J].
CLERMONT, S ;
CORBIER, C ;
MELY, Y ;
GERARD, D ;
WONACOTT, A ;
BRANLANT, G .
BIOCHEMISTRY, 1993, 32 (38) :10178-10184