Continuous biospecific affinity purification of enzymes by simulated moving-bed chromatography - Theoretical description and experimental results

被引：22

作者：

Gottschlich, N ^{[1
]}

Weidgen, S ^{[1
]}

Kasche, V ^{[1
]}

机构：

[1] TECH UNIV HAMBURG,D-21071 HAMBURG,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 719卷 / 02期

关键词：

simulated moving-bed chromatography; affinity adsorbents; adsorption isotherms; enzymes; alpha-chymotrypsin; trypsin inhibitor; soybean;

D O I：

10.1016/0021-9673(95)00710-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A system for continuous biospecific affinity purification of enzymes by simulated moving-bed chromatography was designed. The performance of steady-state operation was predicted by a model based on a linear driving force approximation. The system was tested for the purification of bovine alpha-chymotrypsin on immobilized soybean trypsin inhibitor. The theoretical results showed rather good agreement with the experimental results. The recovery was found to be dependent on the extract purity. For higher purities and recoveries the columns must be purged with non-desorbing buffer before shifting from the adsorption to the desorption zone.

引用

页码：267 / 274

页数：8

共 12 条

[1]

[Anonymous], CAN J BIOCH PHYSL

[2]

Bellot J.C., 1991, PROCESS BIOCHEM, V26, P363

[3] SIMULATED COUNTERCURRENT CHROMATOGRAPHY [J].