Xylanase induction by L-sorbose in a fungus, Trichoderma reesei PC-3-7

被引:18
作者
Xu, JP [1 ]
Nogawa, M [1 ]
Okada, H [1 ]
Morikawa, Y [1 ]
机构
[1] Nagaoka Univ Technol, Dept Bioengn, Niigata 9402188, Japan
关键词
xylanase; cellulase; L-sorbose; Trichoderma reesei;
D O I
10.1271/bbb.62.1555
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Xylanase induction by L-sorbose was studied in a resting cell system of a filamentous fungus, Trichoderma reesei PC-3-7, a hypercellulolytic mutant, and compared with that by other inducers. L-Sorbose induced xylanase activity as well as cellulase. It induced a higher level of xylanase activity than sophorose and xylose did. Three main xylanases, xylanase I (Xyn I), xylanase II (Xyn II), and a non-specific endoglucanase I (EG I), were separated using cation-exchange chromatography, and their activity were measured. Xyn II was induced in about the same proportion (60-80% of the total xylanase activity) by all inducers used. On the other hand, Xyn I was apparently induced by L-sorbose, xylose, and xylooligosaccharides, but only a little by sophorose. Northern blot analysis showed that L-sorbose induced Xyn I and Xyn II at the transcriptional level, and more xyn1 mRNA was transcribed after L-sorbose addition than after sophorose. These results suggested that the expressions of both Xyn I and Xyn II are regulated, at least in part, in a different manner. Furthermore, the Xyn I induction by L-sorbose indicated that an unknown common regulatory mechanism may exist between Xyn I and cellulase inductions.
引用
收藏
页码:1555 / 1559
页数:5
相关论文
共 15 条
[1]  
[Anonymous], [No title captured]
[2]   STEREOCHEMISTRY OF THE HYDROLYSIS OF GLYCOSIDIC LINKAGE BY ENDO-BETA-1,4-XYLANASES OF TRICHODERMA-REESEI [J].
BIELY, P ;
KREMNICKY, L ;
ALFOLDI, J ;
TENKANEN, M .
FEBS LETTERS, 1994, 356 (01) :137-140
[3]   THE ENDO-1,4-BETA-GLUCANASE-I FROM TRICHODERMA-REESEI - ACTION ON BETA-1,4-OLIGOMERS AND POLYMERS DERIVED FROM D-GLUCOSE AND D-XYLOSE [J].
BIELY, P ;
VRSANSKA, M ;
CLAEYSSENS, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 200 (01) :157-163
[4]  
COUGHLAN MP, 1993, BIOTECHNOL APPL BIOC, V17, P259
[5]   SPECIFICITY OF CELLULASE AND BETA-XYLANASE INDUCTION IN TRICHODERMA-REESEI QM-9414 [J].
HRMOVA, M ;
BIELY, P ;
VRSANSKA, M .
ARCHIVES OF MICROBIOLOGY, 1986, 144 (03) :307-311
[6]  
KAWAMORI M, 1986, APPL MICROBIOL BIOT, V24, P449
[7]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[8]   Carbon catabolite repression of xylanase I (xyn1) gene expression in Trichoderma reesei [J].
Mach, RL ;
Strauss, J ;
Zeilinger, S ;
Schindler, M ;
Kubicek, CP .
MOLECULAR MICROBIOLOGY, 1996, 21 (06) :1273-1281
[9]   CLONING, SEQUENCING AND ENHANCED EXPRESSION OF THE TRICHODERMA-REESEI ENDOXYLANASE-II (PI 9) GENE XLN2 [J].
SAARELAINEN, R ;
PALOHEIMO, M ;
FAGERSTROM, R ;
SUOMINEN, PL ;
NEVALAINEN, KMH .
MOLECULAR AND GENERAL GENETICS, 1993, 241 (5-6) :497-503
[10]   2 MAJOR XYLANASES OF TRICHODERMA-REESEI [J].
TENKANEN, M ;
PULS, J ;
POUTANEN, K .
ENZYME AND MICROBIAL TECHNOLOGY, 1992, 14 (07) :566-574