Identification of the protein product of the Coch gene (hereditary deafness gene) as the major component of bovine inner ear protein

被引:84
作者
Ikezono, T
Omori, A
Ichinose, S
Pawankar, R
Watanabe, A
Yagi, T
机构
[1] Nippon Med Sch, Dept Otorhinolaryngol, Bunkyo Ku, Tokyo 1138603, Japan
[2] Mitsubishi Kasei Inst Life Sci, Tokyo, Japan
[3] Nippon Med Sch, Dept Mol Biol, Tokyo 113, Japan
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 2001年 / 1535卷 / 03期
关键词
hereditary hearing impairment; hereditary hearing impairment DFNA9; COCH gene; inner ear; proteome; two-dimensional gel electrophoresis;
D O I
10.1016/S0925-4439(00)00101-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In order to better understand the cause of hereditary hearing impairment, we have performed a proteomic analysis of the inner ear proteins using two-dimensional gel electrophoresis. In the process of analysis, we have found very unique properties of the bovine homologue of the human COCH gene product. The COCH gene is responsible for one of the hereditary hearing impairments, DFNA9, and was recently suggested to be a possible genetic factor contributing to Meniere's disease. The Coch protein constitutes 70% of bovine inner ear proteins and is composed of 16 different protein spots, with charge and size heterogeneity. Heterogeneity of this protein suggests that the Coch gene is processed in several ways, at the transcriptional and/or posttranslational level. Much knowledge has accumulated about the hereditary hearing impairment genes; however, little research has been done regarding the protein products of those genes. This is the first report to characterize the Coch protein. Study of the Coch protein might provide more information on the mechanism of hearing and vestibular disorders. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:258 / 265
页数:8
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