Template DNA ratio can affect detection by genus-specific PCR-Denaturing gradient gel electrophoresis of bacteria present at low abundance in mixed populations

被引：8

作者：

Zhang, L

Danon, S

Grehan, M

Lee, A

Mitchell, H ^{[1
]}

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW, Australia

[2] CSIRO, Div Mol Sci, Sydney, NSW, Australia

来源：

HELICOBACTER | 2005年 / 10卷 / 01期

关键词：

Helicobacter species; DGGE; 16S rRNA;

D O I：

10.1111/j.1523-5378.2005.00294.x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The detection of Helicobacter species by genus-specific polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was compared with that by species-specific PCR in murine intestinal samples. Results suggest that, in samples containing multiple Helicobacter species, genus-specific PCR-DGGE may fail to detect all Helicobacter species present and that this relates to the initial template DNA ratio.

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收藏

页码：80 / 82

页数：3

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