Comparative proteomic profiling of murine skin

被引:58
作者
Huang, CM
Foster, KW
DeSilva, T
Zhang, JF
Shi, ZK
Yusuf, N
Van Kampen, KR
Elmets, CA
Tang, DCC
机构
[1] Univ Alabama Birmingham, Dept Dermatol, Birmingham, AL 35294 USA
[2] Vaxin Inc, Birmingham, AL USA
[3] Univ Alabama Birmingham, Gene Therapy Ctr, Birmingham, AL USA
关键词
proteomics; skin; two-dimensional gel electrophoresis;
D O I
10.1046/j.1523-1747.2003.12327.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Mammalian skin is regularly exposed to different environmental stresses, each of which results in specific compensatory changes in protein expression that can be assessed by proteomic analysis. We have established a reference proteome map of BALB/c murine skin allowing the resolution of greater than 500 protein spots in a single two-dimensional polyacrylamide gel. Forty-four protein spots, corresponding to 28 different cutaneous proteins, were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and the Mascot online database searching algorithm. Twenty-five proteins were expressed at higher levels in the epidermis, whereas only nine were found predominantly in the subepidermal tissues. A subset of protein spots exhibited strain-specific expression. Proteins of diverse function were identified, including those involved in stress response, apoptosis, growth inhibition, the maintenance of structural integrity, translational control, energy metabolism, calcium binding, cholesterol transport, and the scavenging of free radicals. Prohibitin expression was detected cutaneously, with more abundant protein and mRNA levels in the epidermis. Five molecular chaperones including protein di-sulfide isomerase, 78 kDa glucose-regulated protein precursor, heat shock protein 60 (HSP60), HSP70, and HSP27 were also identified. Of these, HSP27 expression was confined mainly to the epidermis, and expression of protein disulfide isomerase was found primarily in the subepidermal tissues. Proteomic analysis of skin following heat or cold shock resulted in increased levels of HSP27, HSP60, and HSP70 suggesting involvement of these chaperones in the cutaneous response mechanism to temperature stress. These data establish numerous reference markers within the proteome map of murine skin and provide an important framework for future efforts aimed at characterization of the epidermal and subepidermal responses to environmental changes.
引用
收藏
页码:51 / 64
页数:14
相关论文
共 81 条
[1]   MOLECULAR-CLONING AND COMPLETE AMINO-ACID-SEQUENCE OF FORM-I PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C [J].
BENNETT, CF ;
BALCAREK, JM ;
VARRICHIO, A ;
CROOKE, ST .
NATURE, 1988, 334 (6179) :268-270
[2]   THE CA-2+-BINDING PROTEIN PARVALBUMIN - MOLECULAR-CLONING AND DEVELOPMENTAL REGULATION OF MESSENGER-RNA ABUNDANCE [J].
BERCHTOLD, MW ;
MEANS, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (05) :1414-1418
[3]   Galectin-7 overexpression is associated with the apoptotic process in UVB-induced sunburn keratinocytes [J].
Bernerd, F ;
Sarasin, A ;
Magnaldo, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11329-11334
[4]   RATIO OF LANGERHANS CELLS TO THY-1+ DENDRITIC EPIDERMAL-CELLS IN MURINE EPIDERMIS INFLUENCES THE INTENSITY OF CONTACT HYPERSENSITIVITY [J].
BIGBY, M ;
KWAN, T ;
SY, MS .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1987, 89 (05) :495-499
[5]   Heat shock protein-chaperoned peptides but not free peptides introduced into the cytosol are presented efficiently by major histocompatibility complex I molecules [J].
Binder, RJ ;
Blachere, NE ;
Srivastava, PK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (20) :17163-17171
[6]   Proteomic analysis of skin irritation reveals the induction of HSP27 by sodium lauryl sulphate in human skin [J].
Boxman, ILA ;
Hensbergen, PJ ;
Van der Schors, RC ;
Bruynzeel, DP ;
Tensen, CP ;
Ponec, M .
BRITISH JOURNAL OF DERMATOLOGY, 2002, 146 (05) :777-785
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   The human keratinocyte two-dimensional gel protein database (update 1995): Mapping components of signal transduction pathways [J].
Celis, JE ;
Rasmussen, HH ;
Gromov, P ;
Olsen, E ;
Madsen, P ;
Leffers, H ;
Honore, B ;
Dejgaard, K ;
Vorum, H ;
Kristensen, DB ;
Ostergaard, M ;
Haunso, A ;
Jensen, NA ;
Celis, A ;
Basse, B ;
Lauridsen, JB ;
Ratz, GP ;
Andersen, AH ;
Walbum, E ;
Kjaergaard, I ;
Andersen, I ;
Puype, M ;
VanDamme, J ;
Vandekerckhove, J .
ELECTROPHORESIS, 1995, 16 (12) :2177-+
[9]   UVB-mediated activation of p38 mitogen-activated protein kinase enhances resistance of normal human keratinocytes to apoptosis by stabilizing cytoplasmic p53 [J].
Chouinard, N ;
Valerie, K ;
Rouabhia, M ;
Huot, J .
BIOCHEMICAL JOURNAL, 2002, 365 (01) :133-145
[10]   Early gene expression profile of human skin to injury using high-density cDNA microarrays [J].
Cole, J ;
Tsou, R ;
Wallace, K ;
Gibran, N ;
Isik, F .
WOUND REPAIR AND REGENERATION, 2001, 9 (05) :360-370