Direct electrochemistry of photosystem I

被引：31

作者：

Kievit, O ^{[1
]}

Brudvig, GW ^{[1
]}

机构：

[1] Yale Univ, Dept Chem, New Haven, CT 06520 USA

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2001年 / 497卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

direct electrochemistry; membrane protein; modified gold electrode; photosystem I;

D O I：

10.1016/S0022-0728(00)00467-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Direct electrochemistry has been developed into a powerful tool for the analysis of soluble proteins and enzymes over the past two decades. In the present study, we have expanded the range of proteins to include a solubilized membrane protein, photosystem I. Taking into account the difficulties caused by the presence of detergent, we have shown it to be possible to obtain an electrochemical signal of the primary electron donor, a chlorophyll dimer called P-700, of photosystem I. A reduction potential of E-1/2 = 490 mV vs. SHE with a peak separation of DeltaE = 100 mV was found, in good agreement with literature values. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：139 / 149

页数：11

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