NETs formed by human neutrophils inhibit growth of the pathogenic mold Aspergillus fumigatus

被引：190

作者：

McCormick, Allison ^{[1
]}

Heesemann, Leonie ^{[1
]}

Wagener, Johannes ^{[1
]}

Marcos, Veronica ^{[2
]}

Hartl, Dominik ^{[2
]}

Loeffler, Jurgen ^{[3
]}

Heesemann, Jurgen ^{[1
]}

Ebel, Frank ^{[1
]}

机构：

[1] Univ Munich, Max Von Pettenkofer Inst, D-80336 Munich, Germany

[2] Univ Munich, Childrens Hosp, D-80336 Munich, Germany

[3] Univ Wurzburg, Inst Hyg & Microbiol, D-97070 Wurzburg, Germany

来源：

MICROBES AND INFECTION | 2010年 / 12卷 / 12-13期

关键词：

Aspergillus fumigatus; NET; Neutrophils; Innate immune response; Fungal infection; EXTRACELLULAR TRAPS; MORPHOLOGY; INFECTION; DEFENSE; YEAST;

D O I：

10.1016/j.micinf.2010.06.009

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Neutrophil extracellular traps (NETs) represent a distinct mechanism to control and eliminate microbial infections Our results show that conidia and germ tubes of the human pathogenic mold Aspergillus fumigatus are able to trigger the formation of NETs Viable fungal cells are not essentially required for this host pathogen interaction Neutrophils engulf conidia and thereby inhibit their germination, a process that is independent of NETosis In the experimental set-up used in this study neutrophils do not kill germ tubes but reduce their polar growth and this inhibition depends on NETs as it can be overcome by the addition of DNase 1 The Zn2+ chelator calprotectin is associated with the Aspergdlus-induced NETs and addition of Zn2+ abrogates the NET-mediated growth inhibition In summary, our data provide evidence that NETs are not sufficient to kill A fumigatus but might be a valuable tool to confine infection (C) 2010 Institut Pasteur Published by Elsevier Masson SAS All rights reserved

引用

页码：928 / 936

页数：9

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