A bioassay using the human hepatoblastoma cell line HepG2 for detecting phototoxicity of furocoumarins

被引：22

作者：

Colombain, M

Goll, V

Muyard, F

Girard, C

Bévalot, F

Richert, L

机构：

[1] Fac Med & Pharm, Equipe Chim Therapeut EA482, Lab Pharmacognosie, F-25030 Besancon, France

[2] Fac Med & Pharm, Equipe Cellules Contractiles EA479, Lab Biol Cellulaire, F-25030 Besancon, France

来源：

PLANTA MEDICA | 2001年 / 67卷 / 07期

关键词：

HepG2 cell line; phototoxicity; cytotoxicity; furocoumarins;

D O I：

10.1055/s-2001-17364

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

We successfully evaluated the human hepatoblastoma cell line HepG2 as a model to assess phototoxicity of coumarins. Five natural furocoumarins were tested and their phototoxic activities, obtained by measuring cell viability in the presence of UV using the MTT test, were as follows: xanthotoxin (8-MOP) much greater than heraclenol = trichoclin = imperatorin much greater than peucedanin, both in growing and confluent cell cultures. This easy-to-perform, miniaturised, quantitative and sensitive method could therefore be used as a primary screening test for phototoxicity of a large number of compounds and plant extracts.

引用

页码：644 / 646

页数：3

共 12 条

[1]

MUTAGENICITY AND TOXICITY OF FUROCOUMARINS - COMPARATIVE INVESTIGATIONS IN 2 TEST SYSTEMS [J].