Enhancement of OspC expression by Borrelia burgdorferi in the presence of tick hemolymph

被引：6

作者：

Johns, RH ^{[1
]}

Sonenshine, DE ^{[1
]}

Hynes, WL ^{[1
]}

机构：

[1] Old Dominion Univ, Dept Biol Sci, Norfolk, VA 23529 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 193卷 / 01期

关键词：

Borrelia burgdorferi; OspC; tick; hemolymph;

D O I：

10.1111/j.1574-6968.2000.tb09415.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Borrelia burgdorferi B31 cultured at 28 degreesC was used to assay the effects of tick hemolymph on outer surface protein C (OspC) surface expression. Using immunofluorescence, OspC surface expression was shown to increase in cultures treated with hemolymph from both bodes scapularis and Dermacentor variabilis, while control samples (non-hemolymph treated) showed no increase in the levels of OspC. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis also showed an increase in OspC from spirochetes exposed to tick hemolymph. In addition, a lower-molecular-mass protein band of unknown identity was also enhanced in the hemolymph-exposed spirochetes. The significance of these effects of tick hemolymph on B. burgdorferi outer surface protein expression is unknown. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：137 / 141

页数：5

共 12 条

[1] CHARACTERIZATION OF BORRELIA-BURGDORFERI STRAINS ISOLATED FROM IXODES-PACIFICUS TICKS IN CALIFORNIA [J].

BISSETT, ML ;

HILL, W .

JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (12) :2296-2301

[2] THERMOREGULATION OF PROTEIN-SYNTHESIS IN BORRELIA-BURGDORFERI [J].

CLUSS, RG ;

BOOTHBY, JT .

INFECTION AND IMMUNITY, 1990, 58 (04) :1038-1042

[3] Plasminogen is required for efficient dissemination of B-burgdorferi in ticks and for enhancement of spirochetemia in mice [J].

Coleman, JL ;

Gebbia, JA ;

Piesman, J ;

Degen, JL ;

Bugge, TH ;

Benach, JL .

CELL, 1997, 89 (07) :1111-1119

[4] GROWTH AND MIGRATION OF BORRELIA-BURGDORFERI IN IXODES TICKS DURING BLOOD-FEEDING [J].

DESILVA, AM ;

FIKRIG, E .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1995, 53 (04) :397-404

[5] Inhibition of Borrelia burgdorferi migration from the midgut to the salivary glands following feeding by ticks on OspC-immunized mice [J].

Gilmore, RD ;

Piesman, J .

INFECTION AND IMMUNITY, 2000, 68 (01) :411-414

[6] Response of the tick Dermacentor variabilis (Acari: Ixodidae) to hemocoelic inoculation of Borrelia burgdorferi (Spirochetales) [J].

Johns, R ;

Sonenshine, DE ;

Hynes, WL .

JOURNAL OF MEDICAL ENTOMOLOGY, 2000, 37 (02) :265-270

[7] Borrelia burgdorferi in tick cell culture modulates expression of outer surface proteins A and C in response to temperature [J].

Obonyo, M ;

Munderloh, UG ;

Fingerle, V ;

Wilske, B ;

Kurtti, TJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (07) :2137-2141

[8]

OLIVER JHJ, 1974, J PARASITOL, V58, P375

[9] INDUCTION OF AN OUTER SURFACE PROTEIN ON BORRELIA-BURGDORFERI DURING TICK FEEDING [J].

SCHWAN, TG ;

PIESMAN, J ;

GOLDE, WT ;

DOLAN, MC ;

ROSA, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (07) :2909-2913

[10]

Schwan TG, 2000, J CLIN MICROBIOL, V38, P382

← 1 2 →