Genotoxic and epigenotoxic effects of fine particulate matter from rural and urban sites in Lebanon on human bronchial epithelial cells

被引：73

作者：

Borgie, Mireille ^{[1
,2
,4
]}

Ledoux, Frederic ^{[1
,4
]}

Verdin, Anthony ^{[1
,4
]}

Cazier, Fabrice ^{[3
,4
]}

Greige, Helene ^{[2
,6
]}

Shirali, Pirouz ^{[1
,4
]}

Courcot, Dominique ^{[1
,4
]}

Dagher, Zeina ^{[2
,5
]}

机构：

[1] Univ Littoral Cote dOpale, Maison Rech Environm Ind, UCEIV, F-59140 Dunkerque, France

[2] Univ Libanaise, Grp Rech Mol Bioact, Ecole Doctorale Sci & Technol, Beirut, Lebanon

[3] Univ Littoral Cote dOpale, Maison Rech Environm Ind 1, Ctr Commun Mesures, F-59140 Dunkerque, France

[4] Univ Lille Nord France, Lille, France

[5] Univ Libanaise, Fac Sci, Dept Biol, Beirut, Lebanon

[6] Univ Libanaise, Fac Sci, Dept Chim & Biochim, Beirut, Lebanon

来源：

ENVIRONMENTAL RESEARCH | 2015年 / 136卷

关键词：

Airborne particulate matter; Lebanon; Physicochemical characterization; BEAS-2B cells; Genotoxicity; Epigenotoxicity; Lung cancer; POLYCYCLIC-AROMATIC-HYDROCARBONS; EASTERN MEDITERRANEAN CITY; AIR-POLLUTION; LUNG-CANCER; CHEMICAL-COMPOSITION; OXIDATIVE DAMAGE; CIGARETTE-SMOKE; DNA-DAMAGE; TELOMERASE ACTIVITY; RECEPTOR REPRESSOR;

D O I：

10.1016/j.envres.2014.10.010

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

Assessment of air pollution by particulate matter (PM) is strongly required in Lebanon in the absence of an air quality law including updated air quality standards. Using two different PM2.5-03 samples collected at an urban and a rural site, we examined genotoxic/epigenotoxic effects of PM exposure within a human bronchial epithelial cell line (BEAS-2B). Inorganic and organic contents evidence the major contribution of traffic and generating sets in the PM2.5-0.3 composition. Urban PM2.5-0.3 sample increased the phosphorylation of H2AX, the telomerase activity and the miR-21 up-regulation in BEAS-2B cells in a dose-dependent manner. Furthermore, urban PM2.5-0.3 induced a significant increase in CYP1A1, CYP1B1 and AhRR genes expression. The variable concentrations of transition metals and organic compounds detected in the collected PM2.5-0.3 samples might be the active agents leading to a cumulative DNA damage, critical for carcinogenesis. (C) 2014 Elsevier Inc. All rights reserved.

引用

收藏

页码：352 / 362

页数：11

相关论文

共 99 条

[1]

Polycyclic aromatic hydrocarbons within airborne particulate matter (PM2.5) produced DNA bulky stable adducts in a human lung cell coculture model [J].

Abbas, Imane ;

Garcon, Guillaume ;

Saint-Georges, Francoise ;

Andre, Veronique ;

Gosset, Pierre ;

Billet, Sylvain ;

Le Goff, Jeremie ;

Verdin, Anthony ;

Mulliez, Philippe ;

Sicheld, Francois ;

Shirali, Pirouz .

JOURNAL OF APPLIED TOXICOLOGY, 2013, 33 (02) :109-119

[2]

γH2AX: A potential DNA damage response biomarker for assessing toxicological risk of tobacco products [J].

Albino, Anthony P. ;

Jorgensen, Ellen D. ;

Rainey, Patrick ;

Gillman, Gene ;

Clark, T. Jeffrey ;

Gietl, Diana ;

Zhao, Hong ;

Traganos, Frank ;

Darzynkiewicz, Zbigniew .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2009, 678 (01) :43-52

[3]

Mutagenicity and genotoxicity of PM2.5 issued from an urbano-industrialized area of Dunkerque (France) [J].

Andre, V. ;

Billet, S. ;

Pottier, D. ;

Le Goff, J. ;

Pottier, I. ;

Garcon, G. ;

Shirali, P. ;

Sichel, F. .

JOURNAL OF APPLIED TOXICOLOGY, 2011, 31 (02) :131-138

[4]

MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer [J].

Asangani, I. A. ;

Rasheed, S. A. K. ;

Nikolova, D. A. ;

Leupold, J. H. ;

Colburn, N. H. ;

Post, S. ;

Allgayer, H. .

ONCOGENE, 2008, 27 (15) :2128-2136

[5]

Rapid DNA Methylation Changes after Exposure to Traffic Particles [J].

Baccarelli, Andrea ;

Wright, Robert O. ;

Bollati, Valentina ;

Tarantini, Letizia ;

Litonjua, Augusto A. ;

Suh, Helen H. ;

Zanobetti, Antonella ;

Sparrow, David ;

Vokonas, Pantel S. ;

Schwartz, Joel .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2009, 179 (07) :572-578

[6]

Genotoxic potential of Polycyclic Aromatic Hydrocarbons-coated onto airborne Particulate Matter (PM2.5) in human lung epithelial a549 cells [J].

Billet, Sylvain ;

Abbas, Imane ;

Le Goff, Jeremie ;

Verdin, Anthony ;

Andre, Veronique ;

Lafargue, Paul-Eric ;

Hachimi, Adam ;

Cazier, Fabrice ;

Sichel, Francois ;

Shirali, Pirouz ;

Garcon, Guillaume .

CANCER LETTERS, 2008, 270 (01) :144-155

[7]

Biological activities of organic compounds adsorbed onto ambient air particles:: comparison between the cities of Teplice and Prague during the summer and winter seasons 2000-2001 [J].

Binkovdá, B ;

Cerná, M ;

Pastorková, A ;

Jelínek, R ;

Benes, I ;

Novák, J ;

Srám, RJ .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2003, 525 (1-2) :43-59

[8]

Exposure to Metal-Rich Particulate Matter Modifies the Expression of Candidate MicroRNAs in Peripheral Blood Leukocytes [J].

Bollati, Valentina ;

Marinelli, Barbara ;

Apostoli, Pietro ;

Bonzini, Matteo ;

Nordio, Francesco ;

Hoxha, Miriam ;

Pegoraro, Valeria ;

Motta, Valeria ;

Tarantini, Letizia ;

Cantone, Laura ;

Schwartz, Joel ;

Bertazzi, Pier Alberto ;

Baccarelli, Andrea .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2010, 118 (06) :763-768

[9]

Contrasts in Oxidative Potential and Other Particulate Matter Characteristics Collected Near Major Streets and Background Locations [J].

Boogaard, Hanna ;

Janssen, Nicole A. H. ;

Fischer, Paul H. ;

Kos, Gerard P. A. ;

Weijers, Ernie P. ;

Cassee, Flemming R. ;

van der Zee, Saskia C. ;

de Hartog, Jeroen J. ;

Brunekreef, Bert ;

Hoek, Gerard .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2012, 120 (02) :185-191

[10]

Traffic-related air pollution. A pilot exposure assessment in Beirut, Lebanon [J].

Borgie, Mireille ;

Garat, Anne ;

Cazier, Fabrice ;

Delbende, Agnes ;

Allorge, Delphine ;

Ledoux, Frederic ;

Courcot, Dominique ;

Shirali, Pirouz ;

Dagher, Zeina .

CHEMOSPHERE, 2014, 96 :122-128

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