Development of a stand-alone affinity clean-up for lysergic acid diethylamide in urine

被引:9
作者
Francis, JM [1 ]
Craston, DH [1 ]
机构
[1] LAB GOVT CHEMIST,TEDDINGTON TW11 0LY,MIDDX,ENGLAND
关键词
extraction; affinity chromatography; lysergic acid diethylamide; urine; enzyme linked immunosorbent assay; high-performance liquid chromatography;
D O I
10.1039/an9962100177
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A total analysis scheme for lysergic acid diethylamide (LSD) from human urine is described. A simple ELISA technique led to the development and optimization of an affinity clean-up cartridge, resulting in high purification factors with a single combined extraction/clean-up step. LSD can be measured with a straightforward HPI,C-fluorescence technique, which minimizes operating complexity and process implementation time. The method has been applied to urine containing 0.5 ng ml(-1) LSD, and the ability of high-affinity materials to preconcentrate a sample into a small volume should allow the working range of the procedure to be adjusted as required.
引用
收藏
页码:177 / 182
页数:6
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