The tRNA(Tyr) multigene family of Triticum aestivum: Genome organization, sequence analyses and maturation of intron-containing pre-tRNAs in wheat germ extract

被引：12

作者：

Arends, S ^{[1
]}

Kraus, J ^{[1
]}

Beier, H ^{[1
]}

机构：

[1] UNIV WURZBURG, INST BIOCHEM, BIOZENTRUM, D-97074 WURZBURG, GERMANY

来源：

FEBS LETTERS | 1996年 / 384卷 / 03期

关键词：

genome organization; in vitro transcription; in vitro splicing; Triticum; wheat germ extract; tyrosine tRNA genes;

D O I：

10.1016/0014-5793(96)00313-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Southern analysis of Triticum DNA has revealed that nuclear tRNA(Tyr) genes are dispersed at a minimum of 16 loci in the genome. We have isolated six independent tRNA(Tyr) genes from a Triticum aestivum library in addition to three known members of the Triticum tRNA(Tyr) family. Four of the sequenced tRNA(Tyr) genes code for Triticum tRNA(1)(Tyr) and two code for tRNA(2)(Tyr). Three genes encode tRNAs(Tyr) which carry one or two nucleotide substitutions as compared to the conventional genes. The nine Triticum tRNA(Tyr) genes possess highly conserved intron sequences ranging in size from 12 to 14 nucleotides. A common secondary intron structure with the 5' and 3' splice site loops separated by five base pairs can be formed by all pre-tRNAs(Tyr) which are efficiently spliced in the homologous wheat germ extract.

引用

页码：222 / 226

页数：5

共 33 条

[1] NUCLEAR TRANSFER-RNA TYR GENES ARE HIGHLY AMPLIFIED AT A SINGLE CHROMOSOMAL SITE IN THE GENOME OF ARABIDOPSIS-THALIANA [J].

BEIER, D ;

STANGE, N ;

GROSS, HJ ;

BEIER, H .

MOLECULAR AND GENERAL GENETICS, 1991, 225 (01) :72-80

[2] EXPRESSION OF VARIANT NUCLEAR ARABIDOPSIS TRANSFER RNA(SER) GENES AND PRE-TRANSFER-RNA MATURATION DIFFER IN HELA, YEAST AND WHEAT-GERM EXTRACTS [J].

BEIER, D ;

BEIER, H .

MOLECULAR & GENERAL GENETICS, 1992, 233 (1-2) :201-208

[3] UAG READTHROUGH DURING TMV RNA TRANSLATION - ISOLATION AND SEQUENCE OF 2 TRANSFER RNASTYR WITH SUPPRESSOR ACTIVITY FROM TOBACCO PLANTS [J].

BEIER, H ;

BARCISZEWSKA, M ;

KRUPP, G ;

MITNACHT, R ;

GROSS, HJ .

EMBO JOURNAL, 1984, 3 (02) :351-356

[4] THE MOLECULAR-BASIS FOR THE DIFFERENTIAL TRANSLATION OF TMV RNA IN TOBACCO PROTOPLASTS AND WHEAT-GERM EXTRACTS [J].

BEIER, H ;

BARCISZEWSKA, M ;

SICKINGER, HD .

EMBO JOURNAL, 1984, 3 (05) :1091-1096

[5] AN INTRON-CONTAINING TRNA(ARG) GENE WITHIN A LARGE CLUSTER OF HUMAN TRANSFER-RNA GENES [J].

BOURN, D ;

CARR, T ;

LIVINGSTONE, D ;

MCLAREN, A ;

GODDARD, JP .

DNA SEQUENCE, 1994, 5 (02) :83-92

[6] PSEUDOURIDINE MODIFICATION IN THE TRANSFER RNATYR ANTICODON IS DEPENDENT ON THE PRESENCE, BUT INDEPENDENT OF THE SIZE AND SEQUENCE, OF THE INTRON IN EUKARYOTIC TRANSFER RNATYR GENES [J].

CHOFFAT, Y ;

SUTER, B ;

BEHRA, R ;

KUBLI, E .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (08) :3332-3337

[7] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[8] THE TRANSFER RNATYR MULTIGENE FAMILY OF NICOTIANA-RUSTICA - GENOME ORGANIZATION, SEQUENCE ANALYSES AND EXPRESSION INVITRO [J].

FUCHS, T ;

BEIER, D ;

BEIER, H .

PLANT MOLECULAR BIOLOGY, 1992, 20 (05) :869-878

[9] TRANSCRIPTION BY RNA POLYMERASE-III [J].

GEIDUSCHEK, EP ;

TOCCHINIVALENTINI, GP .

ANNUAL REVIEW OF BIOCHEMISTRY, 1988, 57 :873-914

[10]

GREEN CJ, 1990, J BIOL CHEM, V265, P12139

← 1 2 3 4 →