A simple, rapid, and highly efficient gene expression system for multiheme cytochromes c

被引：29

作者：

Ozawa, K

Yasukawa, F

Fujiwara, Y

Akutsu, H ^{[1
]}

机构：

[1] Yokohama Natl Univ, Fac Engn, Dept Chem & Biotechnol, Hodogaya Ku, Yokohama, Kanagawa 2408501, Japan

[2] Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 01期

关键词：

multiheme cytochrome c; Shewanella oneidensis; pUC-type vector; electroporation;

D O I：

10.1271/bbb.65.185

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genes of tetraheme cytochrome c(3) and hexadecaheme high-molecular-weight cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in Shewanella oneidensis TSP-C using pUC-type vectors of E. coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation. The yields of the recombinant proteins in this expression system were much higher than the previously reported ones.

引用

收藏

页码：185 / 189

页数：5

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