Isolation of markers from recently transposed LINE-1 retrotransposons

被引:16
作者
Cantrell, MA [1 ]
Grahn, RA [1 ]
Scott, L [1 ]
Wichman, HA [1 ]
机构
[1] Univ Idaho, Dept Biol Sci, Moscow, ID 83844 USA
关键词
D O I
10.2144/00296rr02
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Many previous techniques for the isolation of endogenous retroelements such as LINE-I retrotransposons have produced major sampling bias or required laborious procedures. These problems led to the isolation of only older elements in some cases. In other cases, specialized systems there required for the isolation of recently transposed elements. We report here a system for the easy isolation of markers from a wide range of LINE-1 elements and the screening of recently transposed elements from that population. This is accomplished by the use of PCR with degenerate primers specific for conserved regions of the reverse transcriptase gene, a modified screening vector and a refined blue/white colony assay that screens for amplified DNA containing open reading frames This method should be applicable to searches for endogenous retroviruses.
引用
收藏
页码:1310 / +
页数:6
相关论文
共 22 条
[1]  
[Anonymous], MOBILE DNA
[2]  
Ausubel FA, 1995, CURRENT PROTOCOLS MO
[3]  
Bénit L, 1999, J VIROL, V73, P3301
[4]  
Casavant NC, 1996, GENETICS, V142, P1289
[5]  
Casavant NC, 2000, GENETICS, V154, P1809
[6]   Rapid amplification of a retrotransposon subfamily is evolving the mouse genome [J].
DeBerardinis, RJ ;
Goodier, JL ;
Ostertag, EM ;
Kazazian, HH .
NATURE GENETICS, 1998, 20 (03) :288-290
[7]  
Friedlander A, 1999, CRIT REV ONCOGENESIS, V10, P129
[8]   DNA FOSSILS AND PHYLOGENETIC ANALYSIS - USING L1 (LINE-1, LONG INTERSPERSED REPEATED) DNA TO DETERMINE THE EVOLUTIONARY HISTORY OF MAMMALS [J].
FURANO, AV ;
USDIN, K .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (43) :25301-25304
[9]   Constructing primate phylogenies front ancient retrovirus sequences [J].
Johnson, WE ;
Coffin, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (18) :10254-10260
[10]   THE EVOLUTION OF COEXISTING HIGHLY DIVERGENT LINE-1 SUBFAMILIES WITHIN THE RODENT GENUS PEROMYSCUS [J].
KASS, DH ;
BERGER, FG ;
DAWSON, WD .
JOURNAL OF MOLECULAR EVOLUTION, 1992, 35 (06) :472-485