Virus induction of heat shock protein 70 reflects a general response to protein accumulation in the plant cytosol

被引:98
作者
Aparicio, F
Thomas, CL
Lederer, C
Niu, Y
Wang, DW
Maule, AJ [1 ]
机构
[1] John Innes Ctr, Norwich NR4 7UH, Norfolk, England
[2] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
关键词
D O I
10.1104/pp.104.058958
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Different cytoplasmically replicating RNA viruses were shown to induce a specific subset of heat-inducible heat shock protein 70 (HSP70) genes in Arabidopsis (Arabidopsis thaliana). To identify the inducing principle, a promoter::reporter system was developed for the facile analysis of differentially responding Arabidopsis HSP70 genes, by infiltration into Nicotiana benthamiana leaves. Through transient expression of individual viral cistrons or through deletion analysis of a viral replicon, we were unable to identify a unique inducer of HSP70. However, there was a positive correlation between the translatability of the test construct and the differential induction of HSP70. Since these data implied a lack of specificity in the induction process, we also expressed a random series of cytosolically targeted Arabidopsis genes and showed that these also differentially induced HSP70. Through a comparison of different promoter::reporter constructs and through measurements of the steady-state levels of the individual proteins, it appeared that the HSP70 response reflected the ability of the cytosol to sense individual properties of particular proteins when expressed at high levels. This phenomenon is reminiscent of the unfolded protein response observed when the induced accumulation of proteins in the endoplasmic reticulum also induces a specific suite of chaperones.
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页码:529 / 536
页数:8
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共 34 条
[1]   A subclass of plant heat shock cognate 70 chaperones carries a motif that facilitates trafficking through plasmodesmata [J].
Aoki, K ;
Kragler, F ;
Xoconostle-Cázares, B ;
Lucas, WJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (25) :16342-16347
[2]   Virus-induced host gene shutoff in animals and plants [J].
Aranda, M ;
Maule, A .
VIROLOGY, 1998, 243 (02) :261-267
[3]   A heat shock transcription factor in pea is differentially controlled by heat and virus replication [J].
Aranda, MA ;
Escaler, M ;
Thomas, CL ;
Maule, AJ .
PLANT JOURNAL, 1999, 20 (02) :153-161
[4]   Induction of HSP70 and polyubiquitin expression associated with plant virus replication [J].
Aranda, MA ;
Escaler, M ;
Wang, DW ;
Maule, AJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (26) :15289-15293
[5]   Agrobacterium transient expression system as a tool for the isolation of disease resistance genes:: application to the Rx2 locus in potato [J].
Bendahmane, A ;
Querci, M ;
Kanyuka, K ;
Baulcombe, DC .
PLANT JOURNAL, 2000, 21 (01) :73-81
[6]   THE GENOME STRUCTURE OF TURNIP CRINKLE VIRUS [J].
CARRINGTON, JC ;
HEATON, LA ;
ZUIDEMA, D ;
HILLMAN, BI ;
MORRIS, TJ .
VIROLOGY, 1989, 170 (01) :219-226
[7]   A cysteine-rich plant protein potentiates Potyvirus movement through an interaction with the virus genome-linked protein VPg [J].
Dunoyer, P ;
Thomas, C ;
Harrison, S ;
Revers, F ;
Maule, A .
JOURNAL OF VIROLOGY, 2004, 78 (05) :2301-2309
[8]   Pea embryonic tissues show common responses to the replication of a wide range of viruses [J].
Escaler, M ;
Aranda, MA ;
Thomas, CL ;
Maule, AJ .
VIROLOGY, 2000, 267 (02) :318-325
[9]   TURNIP CRINKLE VIRUS GENES REQUIRED FOR RNA REPLICATION AND VIRUS MOVEMENT [J].
HACKER, DL ;
PETTY, ITD ;
WEI, N ;
MORRIS, TJ .
VIROLOGY, 1992, 186 (01) :1-8
[10]   Complex spatial responses to cucumber mosaic virus infection in susceptible Cucurbita pepo cotyledons [J].
Havelda, Z ;
Maule, AJ .
PLANT CELL, 2000, 12 (10) :1975-1985