In vitro study of prebiotic properties of Levan-type exopolysaccharides from lactobacilli and non-digestible carbohydrates using denaturing gradient gel electrophoresis

被引：167

作者：

Dal Bello, F ^{[1
]}

Walter, J ^{[1
]}

Hertel, C ^{[1
]}

Hammes, WP ^{[1
]}

机构：

[1] Univ Hohenheim, Inst Food Technol, D-70599 Stuttgart, Germany

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2001年 / 24卷 / 02期

关键词：

exopolysaccharide; Lactobacillas sanfranciscensis; fructooligosaccharide; DGGE; prebiotics; intestinal flora;

D O I：

10.1078/0723-2020-00033

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Batch cultures inoculated with human faeces were used to study the prebiotic properties of levan-type exopolysaccharides (EPS) from Lactobacillus sanfranciscensis as well as levan, inulin, and fructooligosaccharide (FOS). Denaturing gradient gel electrophoresis of 16S rDNA fragments generated by PCR with universal primers was used to analyse the cultures. Characteristic changes were revealed in the composition of the gut bacteria during fermentation of the carbohydrates. An enrichment of Bifidobacterium spp. was found for the EPS and inulin but not for levan and FOS. The bifidogenic effect of the EPS was confirmed by culturing on selective medium. In addition, the use of EPS and FOS resulted in enhanced growth of Eubacterium biforme and Clostridium perfringens, respectively.

引用

页码：232 / 237

页数：6

共 25 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] Microbial community dynamics during production of the Mexican fermented maize dough pozol [J].

ben Omar, N ;

Ampe, F .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (09) :3664-3673

[3]

Crittenden RG, 1999, PROBIOTICS: A CRITICAL REVIEW, P141

[4] Heteropolysaccharides from lactic acid bacteria [J].

De Vuyst, L ;

Degeest, B .

FEMS MICROBIOLOGY REVIEWS, 1999, 23 (02) :153-177

[5] Prebiotics, probiotics and human gut microbiology [J].

Fooks, LJ ;

Fuller, R ;

Gibson, GR .

INTERNATIONAL DAIRY JOURNAL, 1999, 9 (01) :53-61

[6] Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use [J].

Gibson, GR ;

Fuller, R .

JOURNAL OF NUTRITION, 2000, 130 (02) :391S-395S

[7] ENRICHMENT OF BIFIDOBACTERIA FROM HUMAN GUT CONTENTS BY OLIGOFRUCTOSE USING CONTINUOUS-CULTURE [J].

GIBSON, GR ;

WANG, X .

FEMS MICROBIOLOGY LETTERS, 1994, 118 (1-2) :121-127

[8] SELECTIVE STIMULATION OF BIFIDOBACTERIA IN THE HUMAN COLON BY OLIGOFRUCTOSE AND INULIN [J].

GIBSON, GR ;

BEATTY, ER ;

WANG, X ;

CUMMINGS, JH .

GASTROENTEROLOGY, 1995, 108 (04) :975-982

[9] DIETARY MODULATION OF THE HUMAN COLONIC MICROBIOTA - INTRODUCING THE CONCEPT OF PREBIOTICS [J].

GIBSON, GR ;

ROBERFROID, MB .

JOURNAL OF NUTRITION, 1995, 125 (06) :1401-1412

[10]

Hammes W.P., 1998, Microbiology of Fermented Foods, V1, P199, DOI 10.1007/978-1-4613-0309-1_8

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