Diversity and characterization of sulfate-reducing bacteria in groundwater at a uranium mill tailings site

被引:164
作者
Chang, YJ
Peacock, AD
Long, PE
Stephen, JR
McKinley, JP
Macnaughton, SJ
Hussain, AKMA
Saxton, AM
White, DC
机构
[1] Univ Tennessee, Ctr Biomarker Anal, Knoxville, TN 37932 USA
[2] Univ Tennessee, Dept Anim Sci, Knoxville, TN 37932 USA
[3] Pacific NW Natl Lab, Environm Technol, Richland, WA 99352 USA
[4] Hort Res Int, Crop & Weed Sci, Warwick CV35 9EF, England
[5] AEA Technol Environm, Abingdon OX14 3BD, Oxon, England
关键词
D O I
10.1128/AEM.67.7.3149-3160.2001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microbially mediated reduction and immobilization of U(VI) to U(TV) plays a role in both natural attenuation and accelerated bioremediation of uranium contaminated sites. To realize bioremediation potential and accurately predict natural attenuation, it is important to first understand the microbial diversity of such sites. In this paper, the distribution of sulfate-reducing bacteria (SRB) in contaminated groundwater associated with a uranium mill tailings disposal site at Shiprock, N.Mex,, was investigated. Two culture-independent analyses were employed: sequencing of clone libraries of PCR-amplified dissimilatory sulfite reductase (DSR) gene fragments and phospholipid fatty acid (PLFA) biomarker analysis. A remarkable diversity among the DSR sequences was revealed, including sequences from F-Proteobacteria, gram-positive organisms, and the Nitrospira division. PLFA analysis detected at least,52 different mid-chain-branched saturate PLFA and included a high proportion of 10me16:0, Desulfotomaculum and Desulfotomaculum-like sequences were the most dominant DSR genes detected. Those belonging to SRB within F-Proteobacteria were mainly recovered from low-uranium (less than or equal to 302 ppb) samples. One Desulfotomaculum like sequence cluster overwhelmingly dominated high-U (>1,500 ppb) sites. Logistic regression showed a significant influence of uranium concentration over the dominance of this cluster of sequences (P = 0.0001), This strong association indicates that Desulfotomaculum has remarkable tolerance and adaptation to high levels of uranium and suggests the organism's possible involvement in natural attenuation of uranium. The in situ activity level of Desulfotomaculum in uranium-contaminated environments and its comparison to the activities of other SRB and other functional groups should be an important area for future research.
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页码:3149 / 3160
页数:12
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