Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30

被引：11

作者：

Beis, K

Nesper, J

Whitfield, C

Naismith, JH ^{[1
]}

机构：

[1] Univ St Andrews, Ctr Biomol Sci, St Andrews KY16 9ST, Fife, Scotland

[2] Univ Guelph, Dept Microbiol, Guelph, ON N1G 2W1, Canada

[3] Univ Halle, Physiol Inst Katschinski NBL3 NG3, Halle Saale, Germany

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷

关键词：

D O I：

10.1107/S0907444903029494

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E. coli; closely related homologues are found in other bacteria that produce extracellular polysaccharides. The Wza crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a=94.6, b=215.5, c=218.5 Angstrom. A data set to 3.0 Angstrom with 99.8% completeness and an R-merge of 10.5% has been collected from a single crystal.

引用

页码：558 / 560

页数：3

共 15 条

[1] Gene products required for surface expression of the capsular form of the group 1 K antigen in Escherichia coli (09a:K30) [J].