Construction and Evaluation of Hemoglobin-Based Capsules as Blood Substitutes

被引：98

作者：

Jia, Yi ^{[1
]}

Cui, Yue ^{[1
]}

Fei, Jinbo ^{[1
]}

Du, Mingchun ^{[1
]}

Dai, Luru ^{[2
]}

Li, Junbai ^{[1
,2
]}

Yang, Yang ^{[2
]}

机构：

[1] Chinese Acad Sci, Inst Chem, CAS Key Lab Colloid & Interface Sci, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

[2] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China

来源：

ADVANCED FUNCTIONAL MATERIALS | 2012年 / 22卷 / 07期

关键词：

capsules; hemoglobin; biocompatibility; hemocompatibility; blood substitutes; CROSS-LINKED HEMOGLOBIN; OXYGEN CARRIERS; AUTOFLUORESCENT PROPERTIES; CARBONATE MICROPARTICLES; ENCAPSULATED HEMOGLOBIN; SYNTHETIC ERYTHROCYTES; HEPARIN; HEMOCOMPATIBILITY; RATS; NANOPARTICLES;

D O I：

10.1002/adfm.201102737

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Hemoglobin-based capsules for use as blood substitutes are successfully fabricated by covalent layer-by-layer assembly. Dialdehyde heparin (DHP) is used both as one of the wall components and a cross-linker without employing other extraneous or toxic crosslinking agents. The biocompatibility of (Hb/DHP)6 microcapsules is evaluated through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay and cell experiments. The hemocompatibility of (Hb/DHP)6 microcapsules is characterized in terms of prothrombin time, thrombin time, activated partial thromboplastin time, and hemolysis rate. The oxygen-carrying capacity of the microcapsules is demonstrated by converting the deoxy-Hb state of the microcapsules into the oxy-Hb state. All these results demonstrate that the hemoglobin-based microcapsules exhibit oxygen-carrying capacity as well as biocompatibility and hemocompatility, indicating that the as-prepared capsules have great potential to function as blood substitutes.

引用

页码：1446 / 1453

页数：8

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