Mutagenic potential of stereoisomeric bay region (+)- and (-)-cis-anti-benzo[a]pyrene diol epoxide-N2-2′-deoxyguanosine adducts in Escherichia coli and simian kidney cells

被引:84
作者
Fernandes, A
Liu, TM
Amin, S
Geacintov, NE
Grollman, AP
Moriya, M [1 ]
机构
[1] SUNY Stony Brook, Dept Pharmacol Sci, Stony Brook, NY 11794 USA
[2] NYU, Dept Chem, New York, NY 10003 USA
[3] Amer Hlth Fdn, Valhalla, NY 10595 USA
关键词
D O I
10.1021/bi980401f
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated the mutagenic potential of site-specifically positioned DNA adducts with (+)- and (-)-cis-anti stereochemistry derived from the binding of r7, t8-dihydroxy-t9, 10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) to N-2-2'-deoxyguanosine (G(1) or G(2)) in the sequence context (5')TCCTCCTG(1) G(2)CCTCTC. BPDE-modified oligodeoxynucleotides were ligated to a single-stranded DNA vector and replicated in Escherichia coli or simian kidney (COS7) cells. The presence of (+)- or (-)-cis adduct strongly reduced the yield of transformants in E. coli, and the yield was improved by the induction of SOS functions. Both adducts were mutagenic in E. coli and COS cells, generating primarily G --> T transversions. In E, coli, the (-)-cis adduct was more mutagenic than the (+)-cis adduct, while in COS cells, both adducts were equally mutagenic. These results were compared with those obtained with stereoisomeric (+)- and (-)-trans adducts [Moriya, M., et al. (1996) Biochemistry 35, 16646-16651). In E, coli, cis adducts, especially (-)-cis adducts, are consistently more mutagenic than the comparable trans adduct, In COS cells, trans adducts yield higher frequencies of mutations than the two cis adducts and, with the exception of the high-mutation frequency associated with the (+)-trans adduct at G(2), relatively small differences in mutation frequencies are observed for the three other adducts. In E. coli, mutation frequency is a pronounced function of adduct stereochemistry and adduct position. These findings suggest that the fidelity of translesional synthesis across BPDE-dG adducts is strongly influenced by adduct stereochemistry, nucleotide sequence context, and the DNA replication complex.
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页码:10164 / 10172
页数:9
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