Sequence analysis and expression of human neuropsin cDNA and gene

被引：76

作者：

Yoshida, S ^{[1
]}

Taniguchi, M ^{[1
]}

Hirata, A ^{[1
]}

Shiosaka, S ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Div Struct Cell Biol, Nara 6301, Japan

来源：

GENE | 1998年 / 213卷 / 1-2期

关键词：

serine protease; hippocampus; keratinocytes;

D O I：

10.1016/S0378-1119(98)00232-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Neuropsin is a serine protease which is thought to function in a variety of tissues including the brain and skin. This protease has been shown to have important roles in neural plasticity in mice. Here we have cloned a cDNA and analyzed the gene for human neuropsin by polymerase chain reaction-based strategies. The cDNA. had 72% identity to mouse neuropsin. The deduced amino acid sequence showed 72% identity to mouse neuropsin. Key amino acid residues for the enzyme activity and all cysteine residues were conserved between human and mouse neuropsin. The gene for human neuropsin had six exons and five introns, and the gene organization is similar to trypsin-type serine proteases. The mRNA was expressed in primary cultures of keratinocytes. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：9 / 16

页数：8

共 27 条

[1] Effects of oxidative stress on the expression of limbic-specific protease neuropsin and avoidance learning in mice [J].