pRB induces Sp1 activity by relieving inhibition mediated by MDM2

被引：43

作者：

Johnson-Pais, T

Degnin, C

Thayer, MJ

机构：

[1] Oregon Hlth Sci Univ, Vollum Inst, Portland, OR 97201 USA

[2] Oregon Hlth Sci Univ, Dept Mol & Med Genet, Portland, OR 97201 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 05期

关键词：

D O I：

10.1073/pnas.051415898

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

pRB activates transcription by a poorly understood mechanism that involves relieving negative regulation of the promoter specificity factor Sp1. We show here that MDM2 inhibits Sp1-mediated transcription, that MDM2 binds directly to Sp1 in vitro as well as in vivo, and that MDM2 inhibits the DNA-binding activity of Sp1. Forced expression of pRB relieves MDM2-mediated repression, and interaction of pRB with the MDM2-Sp1 complex releases Sp1 and restores DNA binding. These results suggest a model in which the opposing activities of MDM2 and pRB regulate Sp1 DNA-binding and transcriptional activity.

引用

页码：2211 / 2216

页数：6

共 34 条

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