A benchmark study with SealChip™ planar patch-clamp technology

被引：47

作者：

Xu, J ^{[1
]}

Guia, A ^{[1
]}

Rothwarf, D ^{[1
]}

Huang, MX ^{[1
]}

Sithiphong, K ^{[1
]}

Ouang, J ^{[1
]}

Tao, GL ^{[1
]}

Wang, XB ^{[1
]}

Wu, L ^{[1
]}

机构：

[1] AVIVA Biosci Corp, San Diego, CA 92121 USA

来源：

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES | 2003年 / 1卷 / 05期

关键词：

D O I：

10.1089/154065803770381039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Although conventional patch-clamp methods provide high information content, they are labor-intensive and suffer from low throughput and high overall cost. Several approaches for achieving high throughput electrophysiology are under development, among which microchip-based patch-clamp systems uniquely achieve a higher degree of miniaturization, faster perfusion and mixing, and lower reagent cost without losing information content. The goal of this study was to establish a benchmark for our biochip technology with 52 chips tested sequentially. We demonstrate that our microfabrication and processing technology is sufficiently mature to produce a consistent hole size. We further demonstrate high-quality planar whole-cell patch clamping with >75% overall success rate at achieving gigaohm seals, followed by stable whole-cell access lasting at least 15 min with. access resistance (Ra) below 15 MOmega and membrane resistance (Rm) above 200 MOmega. These biochips are ideally suited for high throughput compound screening for ion channel targets.

引用

页码：675 / 684

页数：10

共 16 条

[1] Whole cell patch clamp recording performed on a planar glass chip [J].