Smartphone-enabled optofluidic exosome diagnostic for concussion recovery

被引：72

作者：

Ko, Jina ^{[1
]}

Hemphill, Matthew A. ^{[1
]}

Gabrieli, David ^{[1
]}

Wu, Leon ^{[1
]}

Yelleswarapu, Venkata ^{[1
]}

Lawrence, Gladys ^{[1
]}

Pennycooke, Wesley ^{[1
]}

Singh, Anup ^{[1
]}

Meaney, Dave F. ^{[1
,3
]}

Issadore, David ^{[1
,2
]}

机构：

[1] Univ Penn, Sch Engn & Appl Sci, Dept Bioengn, Philadelphia, PA 19104 USA

[2] Univ Penn, Sch Engn & Appl Sci, Dept Elect & Syst Engn, Philadelphia, PA 19104 USA

[3] Univ Penn, Perelman Sch Med, Dept Neurosurg, Philadelphia, PA 19104 USA

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

TRAUMATIC BRAIN-INJURY; MECHANICAL INJURY; AMPA RECEPTORS; IN-VITRO; PROTEIN; QUANTIFICATION; CONSEQUENCES; BIOMARKERS; FLUID;

D O I：

10.1038/srep31215

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

A major impediment to improving the treatment of concussion is our current inability to identify patients that will experience persistent problems after the injury. Recently, brain-derived exosomes, which cross the blood-brain barrier and circulate following injury, have shown great potential as a noninvasive biomarker of brain recovery. However, clinical use of exosomes has been constrained by their small size (30-100 nm) and the extensive sample preparation (>24 hr) needed for traditional exosome measurements. To address these challenges, we developed a smartphone-enabled optofluidic platform to measure brain-derived exosomes. Sample-to-answer on our chip is 1 hour, 10x faster than conventional techniques. The key innovation is an optofluidic device that can detect enzyme amplified exosome biomarkers, and is read out using a smartphone camera. Using this approach, we detected and profiled GluR2+ exosomes in the post-injury state using both in vitro and murine models of concussion.

引用

页数：12

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