Notch Signaling Activation Enhances Human Adipose-Derived Stem Cell Retinal Differentiation

被引：15

作者：

Huang, Yuqiang ^{[1
,2
]}

Ng, Tsz Kin ^{[1
,2
,3
,4
]}

Chen, Chong-Bo ^{[1
,2
]}

Huang, Bing ^{[1
,2
]}

Liang, Jiajian ^{[1
,2
]}

Pang, Chi Pui ^{[1
,2
,4
]}

Zhang, Mingzhi ^{[1
,2
]}

机构：

[1] Shantou Univ, Joint Shantou Int Eye Ctr, Shantou, Guangdong, Peoples R China

[2] Chinese Univ Hong Kong, Shantou, Guangdong, Peoples R China

[3] Shantou Univ, Coll Med, Shantou, Guandong, Peoples R China

[4] Chinese Univ Hong Kong, Dept Ophthalmol & Visual Sci, Hong Kong, Hong Kong, Peoples R China

来源：

STEM CELLS INTERNATIONAL | 2018年 / 2018卷

关键词：

PROGENITOR CELLS; TISSUE; PROLIFERATION; MAINTENANCE; ANTAGONISM; EXPRESSION; GLUTAMATE; FATE;

D O I：

10.1155/2018/9201374

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Retinal disease treatment by stem cell-based replacement relies on stem cell differentiation into retinal cells. We previously demonstrated that human periodontal ligament-derived stem cells can be directed into retinal lineage upon induction. Here, we report the transdifferentiation potential of human adipose-derived stem cells (ASCs) into retinal lineage and its enhancement by Notch signaling modulation. Human ASCs, isolated from abdominal fat, expressed mesenchymal but not hematopoietic stem cell markers, and they can differentiate into adipocytes, chondrocytes, and osteoblasts in vitro. Upon noggin/Dkk-1/IGF-1 induction, the treated ASCs showed elevated expression of retinal progenitor, retinal ganglion, and photoreceptor cell markers as well as the glutamate-evoked calcium response, which was not observed in the noninduced cells. Compared to the regular induction treatment, Notch signaling activation by JAG1 enhanced the expression of retinal progenitor and precursor markers without affecting the glutamate-evoked calcium response. In contrast, Notch signaling inhibition by DAPT showed more retinal ganglion cells, but delayed the response to glutamate stimulation. In summary, our results revealed that human ASCs possess a retinal transdifferentiation potential upon noggin/Dkk-1/IGF-1 induction, which can further be enhanced by Notch signaling activation.

引用

页数：13

共 39 条

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