Radiosurgery using heavy ion microbeams for biological study:: Fate mapping of the cellular blastoderm-stage egg of the silkworm, Bombyx mori

被引：12

作者：

Kiguchi, K

Shirai, K

Kanekatsu, R

Kobayashi, Y

Tu, ZL

Funayama, T

Watanabe, H

机构：

[1] Shinshu Univ, Fac Text Sci & Technol, Dept Appl Biol, Nagano 3868567, Japan

[2] Japan Atom Energy Res Inst, Dept Ion Beam Appl Biol, Gunma 3701292, Japan

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 2003年 / 210卷

关键词：

heavy ions; microbeam; radio-microsurgery; Bombyx mori; fate map; cellular blastoderm;

D O I：

10.1016/S0168-583X(03)01054-1

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 [仪器科学与技术]; 080401 [精密仪器及机械]; 081102 [检测技术与自动化装置];

摘要：

We investigated the effects of heavy ions on embryogenesis of the silkworm, Bombyx mori using a collimated heavy ion microbeam from the vertical beam line of an AVF-cyclotron. Eggs were exposed to carbon ions at the cellular blastoderm stage. Microbeams were found to be extremely useful for radio-microsurgical inactivation of nuclei or cells in the target site. Spot irradiation caused abnormal embryos, which showed localized defects such as deletion, duplication and fusion, depending on dose, beam size and site of irradiation. The location and frequency of defects on the resultant embryos were closely correlated to the irradiation site. Based on this correlation, a fate map was established for the Bombyx egg at the cellular blastoderm stage. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：312 / 315

页数：4