Severity of the streptomycin resistance and streptomycin dependence phenotypes of ribosomal protein S12 of Thermus thermophilus depends on the identity of highly conserved amino acid residues

被引：25

作者：

Carr, JF ^{[1
]}

Gregory, ST ^{[1
]}

Dahlberg, AE ^{[1
]}

机构：

[1] Brown Univ, Dept Mol Biol Cell Biol & Biochem, Providence, RI 02912 USA

来源：

JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 10期

关键词：

D O I：

10.1128/JB.187.10.3548-3550.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The structural basis for the streptomycin dependence phenotype of ribosomal protein S12 mutants is poorly understood. Here we describe the application of site-directed mutagenesis and gene replacement of Thermus thermophilus rpsL to assess the importance of side chain identity and tertiary interactions as phenotypic determinants of drug-dependent mutants.

引用

页码：3548 / 3550

页数：3

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