Isolation and characterization of a Na+/H+ antiporter gene from the halophyte Atriplex gmelini

被引：222

作者：

Hamada, A ^{[1
]}

Shono, M ^{[1
]}

Xia, T ^{[1
]}

Ohta, M ^{[1
]}

Hayashi, Y ^{[1
]}

Tanaka, A ^{[1
]}

Hayakawa, T ^{[1
]}

机构：

[1] Plantech Res Inst, Aoba Ku, Kanagawa 2270033, Japan

来源：

PLANT MOLECULAR BIOLOGY | 2001年 / 46卷 / 01期

关键词：

AgNHX1; Atriplex gmelini; halophyte; Na+/H+ antiporter; salt stress; vacuole;

D O I：

10.1023/A:1010603222673

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

With a homologous gene region we successfully isolated a Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini, and named it AgNHX1. The isolated cDNA is 2607 bp in length and contains one open reading frame, which comprises 555 amino acid residues with a predicted molecular mass of 61.9 kDa. The amino acid sequence of the AgNHX1 gene showed more than 75% identity with those of the previously isolated NHX1 genes from glycophytes, Arabidopsis thaliana and Oryza sativa. The migration pattern of AgNHX1 was shown to correlate with H+-pyrophosphatase and not with P-type H+-ATPase, suggesting the localization of AgNHX1 in a vacuolar membrane. Induction of the AgNHX1 gene was observed by salt stress at both mRNA and protein levels. The expression of the AgNHX1 gene in the yeast mutant, which lacks the vacuolar-type Na+/H+ antiporter gene (NHX1) and has poor viability under the high-salt conditions, showed partial complementation of the NHX1 functions. These results suggest the important role of the AgNHX1 products for salt tolerance.

引用

页码：35 / 42

页数：8

共 30 条

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[2] Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis [J].