Roles of SWI/SNF and HATs throughout the dynamic transcription of a yeast glucose-repressible gene

被引:58
作者
Geng, FQ [1 ]
Laurent, BC [1 ]
机构
[1] SUNY Downstate Med Ctr, Morse Inst Mol Genet, Dept Microbiol & Immunol, Mol & Cellular Biol Program, Brooklyn, NY 11203 USA
关键词
biphasic; Esa1; Gcn5; stress; SWI/SNF;
D O I
10.1038/sj.emboj.7600035
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eucaryotic gene expression requires chromatin-remodeling activities. We show by time-course studies that transcriptional induction of the yeast glucose-regulated SUC2 gene is rapid and shows a striking biphasic pattern, the first phase of which is partly mediated by the general stress transcription factors Msn2p/Msn4p. The SWI/ SNF ATP-dependent chromatin-remodeling complex associates with the promoter in a similar biphasic manner and is essential for both phases of transcription. Two different histone acetyltransferases, Gcn5p and Esa1p, enhance the binding of SWI/ SNF to the promoter during early transcription and are required for optimal SUC2 induction. Gcn5p is recruited to SUC2 simultaneously with SWI/ SNF, whereas Esa1p associates constitutively with the promoter. This study reveals an unusual transcription pattern of a metabolic gene and suggests a novel strategy by which distinct chromatin remodelers cooperate for the dynamic activation of transcription.
引用
收藏
页码:127 / 137
页数:11
相关论文
共 64 条
[1]   Deciphering the transcriptional histone acetylation code for a human gene [J].
Agalioti, T ;
Chen, GY ;
Thanos, D .
CELL, 2002, 111 (03) :381-392
[2]   Ordered recruitment of chromatin modifying and general transcription factors to the IFN-β promoter [J].
Agalioti, T ;
Lomvardas, S ;
Parekh, B ;
Yie, JM ;
Maniatis, T ;
Thanos, D .
CELL, 2000, 103 (04) :667-678
[3]   Control by nutrients of growth and cell cycle progression in budding yeast, analyzed by double-tag flow cytometry [J].
Alberghina, L ;
Smeraldi, C ;
Ranzi, BM ;
Porro, D .
JOURNAL OF BACTERIOLOGY, 1998, 180 (15) :3864-3872
[4]   NuA4, an essential transcription adaptor/histone H4 acetyltransferase complex containing Esa1p and the ATM-related cofactor Tra1p [J].
Allard, S ;
Utley, RT ;
Savard, J ;
Clarke, A ;
Grant, P ;
Brandl, CJ ;
Pillus, L ;
Workman, JL ;
Côté, J .
EMBO JOURNAL, 1999, 18 (18) :5108-5119
[5]   ACETYLATION + METHYLATION OF HISTONES + THEIR POSSIBLE ROLE IN REGULATION OF RNA SYNTHESIS [J].
ALLFREY, VG ;
FAULKNER, R ;
MIRSKY, AE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1964, 51 (05) :786-+
[6]   Glucose depletion rapidly inhibits translation initiation in yeast [J].
Ashe, MP ;
De Long, SK ;
Sachs, AB .
MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (03) :833-848
[7]  
Ausubel FM, 1995, CURRENT PROTOCOLS MO
[8]   Increasing the rate of chromatin remodeling and gene activation -: a novel role for the histone acetyltransferase Gcn5 [J].
Barbaric, S ;
Walker, J ;
Schmid, A ;
Svejstrup, JQ ;
Hörz, W .
EMBO JOURNAL, 2001, 20 (17) :4944-4951
[9]   Continuous and widespread roles for the Swi-Snf complex in transcription [J].
Biggar, SR ;
Crabtree, GR .
EMBO JOURNAL, 1999, 18 (08) :2254-2264
[10]   CHARACTERIZATION OF SACCHAROMYCES-CEREVISIAE GENES ENCODING SUBUNITS OF CYCLIC AMP-DEPENDENT PROTEIN-KINASE [J].
CANNON, JF ;
TATCHELL, K .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2653-2663