Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

被引：122

作者：

Yang, Cheng ^{[1
,2
]}

Lates, Vasilica ^{[3
]}

Prieto-Simon, Beatriz ^{[4
]}

Marty, Jean-Louis ^{[3
]}

Yang, Xiurong ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Elect Chem, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] Univ Perpignan, IMAGES EA 4218, F-66860 Perpignan, France

[4] Inst Bioengn Catalunya IBEC, Barcelona 08028, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2012年 / 32卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Ochratoxin A; Aptamer; G-quadruplex; DNAzyme; Hairpin; LINKED-IMMUNOSORBENT-ASSAY; G-QUADRUPLEX APTAMERS; SINGLE-STRANDED-DNA; MOLECULES; SELECTION; ENZYME; INVITRO; LIGANDS; BIND;

D O I：

10.1016/j.bios.2011.12.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10 nM, showing a limit of detection of 2.5 nM. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：208 / 212

页数：5

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