Pseudoazurin mediates periplasmic electron flow in a mutant strain of Paracoccus denitrificans lacking cytochrome c550

被引：21

作者：

Koutny, M

Kucera, I

Tesarík, R

Turánek, J

Van Spanning, RJM

机构：

[1] Masaryk Univ, Fac Sci, Dept Biochem, CZ-61137 Brno, Czech Republic

[2] Vet Res Inst, Dept Immunol, CZ-62132 Brno, Czech Republic

[3] Free Univ Amsterdam, Fac Biol, Dept Mol Cell Physiol, NL-1081 HV Amsterdam, Netherlands

来源：

FEBS LETTERS | 1999年 / 448卷 / 01期

关键词：

pseudoazurin; nitrite reduction; electron transport; Paracoccus denitrificans;

D O I：

10.1016/S0014-5793(99)00345-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A periplasmic protein able to transfer electrons from cytoplasmic membrane to the periplasmic nitrite reductase (cytochrome cd(1)) has been purified from the anoxically grown cytochrome C-550 mutant strain Pd2121 and shown to be pseudoazurin by several independent criteria (molecular mass, copper content, visible spectrum, N-terminal amino acid sequence). Under our assay conditions, the half-saturation of electron transport occurred at about 10 mu M pseudoazurin; the reaction was retarded by increasing ionic strength. (C) 1999 Federation of European Biochemical Societies.

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收藏

页码：157 / 159

页数：3

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