Protein folding observed by capillary electrophoresis in isoelectric buffers

被引:28
作者
Stellwagen, E [1 ]
Gelfi, C
Righetti, PG
机构
[1] Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA
[2] CNR, Ist Tecnol Biomed Avanzate, I-20090 Milan, Italy
[3] Univ Verona, Dept Agr & Ind Biotechnol, I-37134 Verona, Italy
关键词
protein folding; buffer composition; proteins; cytochromes;
D O I
10.1016/S0021-9673(98)01048-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Capillary zone electrophoresis measurements in acidic isoelectric buffers provide a sensitive and rapid method for comparison of the folding and stability of wild type, mutant or post-translationally modified proteins. The potential of the method is illustrated using the small globular protein cytochrome c. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:131 / 138
页数:8
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