Preparation of mixed matrix adsorber membranes for protein recovery

This paper presents a generic technology allowing the incorporation of functional entities into a porous substrate. Various ion exchange particles were incorporated into an ethylene vinyl alcohol (EVAL) copolymer porous matrix by an immersion phase separation process and a heterogeneous matrix, composed of solid particles surrounded by a polymeric film, was formed. The developed concept is flexible and offers the possibility to easily adjust the geometry, the adsorption capacity, as well as the functionality of the structure. A series of fibers as well as flat membranes bearing an adsorptive function and high protein binding capacities were prepared. The membranes were characterized with respect to their morphology, porosity, permeability and adsorption capacity. High values of the protein adsorption capacity (40-45 mg bovine serum albumin (BSA)/ml membrane) were obtained by static adsorption experiments. In a sequential desorption step by changing the pH and/or the ionic strength of the eluent, up to 90% protein recovery can be obtained. Dynamic capacity experiments were performed by flowing the protein solution through a stack of 10 membranes. The protein mass adsorbed per unit of membrane bed was calculated at a breakthrough concentration 10% of the feed concentration. The adsorber membranes can be reused in multiple adsorption/desorption cycles without significant loss of performance. (C) 2003 Elsevier Science B.V. All rights reserved.