Heterogeneity in single DNA conformational fluctuations

被引：21

作者：

Edman, L ^{[1
]}

Wennmalm, S ^{[1
]}

Tamsen, F ^{[1
]}

Rigler, R ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biophys, S-17177 Stockholm, Sweden

来源：

CHEMICAL PHYSICS LETTERS | 1998年 / 292卷 / 1-2期

关键词：

D O I：

10.1016/S0009-2614(98)00633-2

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Non-exponential behaviour in bulk measurements of two-state molecular reactions can have different origins. The probability density for the time which one of two states is occupied during different observation interval times can be used to reveal system characteristics if single molecules are studied. An evaluation of new experimental data from single DNA molecules labeled with a fluorescent conformation probe proves the heterogeneity of the ensemble system. Also, the anomalicity is concluded to be low for single events. The evaluation method can be applied in other experiments where two-state reversible processes are studied for isolated systems. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：15 / 21

页数：7

共 19 条

[1] CO BINDING TO HEME-PROTEINS - A MODEL FOR BARRIER HEIGHT DISTRIBUTIONS AND SLOW CONFORMATIONAL-CHANGES [J].

AGMON, N ;

HOPFIELD, JJ .

JOURNAL OF CHEMICAL PHYSICS, 1983, 79 (04) :2042-2053

[2] SPIN-GLASSES - EXPERIMENTAL FACTS, THEORETICAL CONCEPTS, AND OPEN QUESTIONS [J].

BINDER, K ;

YOUNG, AP .

REVIEWS OF MODERN PHYSICS, 1986, 58 (04) :801-976

[3] Conformational transitions monitored for single molecules in solution [J].

Edman, L ;

Mets, U ;

Rigler, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (13) :6710-6715

[4]

Edman L., 1995, Experimental Technique of Physics, V41, P157

[5] FLUCTUATIONS OF THE PROBABILITY DENSITY OF DIFFUSING PARTICLES FOR DIFFERENT REALIZATIONS OF A RANDOM MEDIUM [J].

EISENBERG, E ;

HAVLIN, S ;

WEISS, GH .

PHYSICAL REVIEW LETTERS, 1994, 72 (18) :2827-2830

[6]

Fischer K. H., 1991, SPIN GLASSES

[7]

FRAUENFELDER H, IN PRESS GLASSES GLA

[8] Two-state dynamics of single biomolecules in solution [J].

Geva, E ;

Skinner, JL .

CHEMICAL PHYSICS LETTERS, 1998, 288 (2-4) :225-229

[9] ANOMALOUS RELAXATION IN THE FRACTAL TIME RANDOM-WALK MODEL [J].

GOMI, S ;

YONEZAWA, F .

PHYSICAL REVIEW LETTERS, 1995, 74 (21) :4125-4128

[10]

GOTZE W, 1991, LIQUIDS FREEZING GLA

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