Mapping Broadly Reactive Norovirus Genogroup I and II Monoclonal Antibodies

被引:16
作者
Crawford, Sue E. [1 ]
Ajami, Nadim [1 ]
Parker, Tracy Dewese [1 ]
Kitamoto, Noritoshi [2 ]
Natori, Katsuro [3 ]
Takeda, Naokazu [3 ]
Tanaka, Tomoyuki [4 ]
Kou, Baijun [1 ]
Atmar, Robert L. [1 ,5 ]
Estes, Mary K. [1 ,5 ]
机构
[1] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
[2] Univ Hyogo, Sch Human Sci & Environm, Kobe, Hyogo 6500044, Japan
[3] Natl Inst Infect Dis, Dept Virol 2, Shinjuku Ku, Tokyo 1628640, Japan
[4] Sakai City Inst Publ Hlth, Sakai, Osaka, Japan
[5] Baylor Coll Med, Dept Med, Houston, TX 77030 USA
关键词
NORWALK-LIKE VIRUSES; BLOOD GROUP ANTIGENS; NURSING-HOMES; FOODBORNE OUTBREAK; UNITED-STATES; GASTROENTERITIS; INFECTION; IDENTIFICATION; EPIDEMIOLOGY; RECOGNITION;
D O I
10.1128/CVI.00520-14
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Noroviruses are responsible for most acute nonbacterial epidemic outbreaks of gastroenteritis worldwide. To develop crossreactive monoclonal antibodies (MAbs) for rapid identification of genogroup I and II (GI and GII) noroviruses (NoVs) in field specimens, mice were immunized with baculovirus-expressed recombinant virus-like particles (VLPs) corresponding to NoVs. Nine MAbs against the capsid protein were identified that detected both GI and GII NoV VLPs. These MAbs were tested in competition enzyme-linked immunosorbent assays (ELISAs) to identify common epitope reactivities to GI and GII VLPs. Patterns of competitive reactivity placed these MAbs into two epitope groups (groups 1 and 2). Epitopes for MAbs NV23 and NS22 (group 1) and MAb F120 (group 2) were mapped to a continuous region in the C-terminal P1 subdomain of the capsid protein. This domain is within regions previously defined to contain cross-reactive epitopes in GI and GII viruses, suggesting that common epitopes are clustered within the P1 domain of the capsid protein. Further characterization in an accompanying paper (B. Kou et al., Clin Vaccine Immunol 22: 160 -167, 2015, http://dx.doi.org/10.1128/CVI.00519-14) revealed that MAb NV23 (epitope group 1) is able to detect GI and GII viruses in stool. Inclusion of the GI and GII cross-reactive MAb NV23 in antigen detection assays may facilitate the identification of GI and GII human noroviruses in stool samples as causative agents of outbreaks and sporadic cases of gastroenteritis worldwide.
引用
收藏
页码:168 / 177
页数:10
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