Electroporation-mediated gene transfer in cardiac tissue

被引：96

作者：

Harrison, RL

Byrne, BJ

Tung, L

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA

[2] Univ Florida, Gene Therapy Ctr, Dept Pediat & Mol Genet & Microbiol, Gainesville, FL 32610 USA

来源：

FEBS LETTERS | 1998年 / 435卷 / 01期

关键词：

electroporation; gene transfer; green fluorescent protein; luciferase; embryonic chick heart;

D O I：

10.1016/S0014-5793(98)00987-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Delivery of genes or macromolecules to cardiovascular tissues provides new therapeutic opportunities for the treatment of many acquired and inherited diseases. To investigate electroporation as a delivery method in cardiac tissue, embryonic chick hearts were studied for uptake of propidium iodide (PI) or DNA encoding either green fluorescent protein (GFP) or luciferase following electrical shock. PI uptake increased monotonically from 6% of heart tissue after 3 shocks to 77% with 12 shocks, GFP and luciferase expression varied in proportion to shock number, with detectable levels in all electrically treated hearts. Thus, electroporation promotes uptake of PI and DNA in cardiac tissue, suggesting further application of this method for therapeutic genes. (C) 1998 Federation of European Biochemical Societies.

引用

页码：1 / 5

页数：5

共 21 条

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