Purification and some properties of wheat germ acid phosphatases

被引：21

作者：

Kawarasaki, Y ^{[1
]}

Nakano, H ^{[1
]}

Yamane, T ^{[1
]}

机构：

[1] NAGOYA UNIV,SCH AGR SCI,DEPT APPL BIOL SCI,LAB MOL BIOTECHNOL,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

来源：

PLANT SCIENCE | 1996年 / 119卷 / 1-2期

关键词：

wheat germ; phosphatase isozymes;

D O I：

10.1016/0168-9452(96)04477-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acid phosphatase isozymes were isolated from wheat germ. Six isozymes were isolated, and one of them was purified to homogeneity. The molecular mass of the purified isozyme was revealed to be 40 kDa (from an SDS-PAGE analysis), or 47 kDa (from a gel filtration analysis), suggesting that the isozyme is a monomer. The N-terminal amino acid sequence of the isozyme was also determined. All of the isozymes showed similar properties; optimum pH, substrate specificities and metal ion inhibition. However, three isozymes were able to hydrolyze phosphotyrosine, but the others were not. Mouse antiserum raised against the purified isozyme could react with other partially purified isozymes except for one isozyme, suggesting that at least five isozymes isolated in this study immunochemically have common epitopes.

引用

页码：67 / 77

页数：11

共 28 条

[1] AKIYAMA T, 1981, PLANT CELL PHYSIOL, V22, P1023
[2] IMMUNOCHEMICAL DIFFERENCES IN ACID-PHOSPHATASE ISOZYMES FROM WHEAT-GERM
AKIYAMA, T
YAMAMOTO, S
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (02): : 437 - 440
[3] ANDERSON CW, 1983, METHOD ENZYMOL, V101, P635
[4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5] BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5
[6] EXOCELLULAR ENZYMES OF CORN ROOTS
CHANG, CW
BANDURSKI, RS
[J]. PLANT PHYSIOLOGY, 1964, 39 (01) : 60 - +
[7] SENSITIVE FLUORESCENT METHOD FOR DETECTION OF GLYCOPROTEINS IN POLYACRYLAMIDE GELS
ECKHARDT, AE
HAYES, CE
GOLDSTEIN, IJ
[J]. ANALYTICAL BIOCHEMISTRY, 1976, 73 (01) : 192 - 197
[8] ACID-PHOSPHATASE-ACTIVITY IN BEAN AND COWPEA PLANTS GROWN UNDER PHOSPHORUS STRESS
FERNANDEZ, DS
ASCENCIO, J
[J]. JOURNAL OF PLANT NUTRITION, 1994, 17 (2-3) : 229 - 241
[9] DIO-9 AND CHLORHEXIDINE - INHIBITORS OF MEMBRANE-BOUND ATPASE AND OF CATION TRANSPORT IN STREPTOCOCCUS FAECALIS
HAROLD, FM
BAARDA, JR
BARON, C
ABRAMS, A
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 183 (01) : 129 - &
[10] ISOLATION AND PARTIAL CHARACTERIZATION OF CYTOPLASMIC AND WALL-BOUND ACID-PHOSPHATASES FROM WHEAT ROOTS
HASEGAWA, Y
LYNN, KR
BROCKBANK, WJ
[J]. CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1976, 54 (10): : 1163 - 1169

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