Poly(3-hydroxybutyrate) depolymerases bind to their substrate by a C-terminal located substrate binding site

被引:47
作者
Behrends, A [1 ]
Klingbeil, B [1 ]
Jendrossek, D [1 ]
机构
[1] UNIV GOTTINGEN, INST MIKROBIOL, D-37077 GOTTINGEN, GERMANY
关键词
biodegradable polyester; poly(3-hydroxybutyrate) depolymerase; substrate-binding site; Pseudomonas lemoignei;
D O I
10.1016/0378-1097(96)00305-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Binding of (i) purified wild-type poly(3-hydroxybutyrate) (PHB) depolymerase PhaZ4 of Pseudomonas lemoignei, (ii) a purified truncated form of PhaZ4, which lacked 55 C-terminal amino acids and (iii) commercial lactate dehydrogenase to aqueous suspensions of PHB, chitin or cellulose was studied. Only the wild-type PHB depolymerase was specifically able to bind to PHB granules. No other combination of protein and polymeric substrate resulted in polymer-bound protein. Similar results were obtained for other PHB depolymerases. We concluded that the C-terminal amino acids of PHB depolymerases represent a PHB-specific binding domain or at least an essential part of it.
引用
收藏
页码:191 / 194
页数:4
相关论文
共 17 条
  • [1] OCCURRENCE, METABOLISM, METABOLIC ROLE, AND INDUSTRIAL USES OF BACTERIAL POLYHYDROXYALKANOATES
    ANDERSON, AJ
    DAWES, EA
    [J]. MICROBIOLOGICAL REVIEWS, 1990, 54 (04) : 450 - 472
  • [2] THE BIOLOGICAL DEGRADATION OF CELLULOSE
    BEGUIN, P
    AUBERT, JP
    [J]. FEMS MICROBIOLOGY REVIEWS, 1994, 13 (01) : 25 - 58
  • [3] BLAAK H, 1995, EUR J BIOCHEM, V229, P132, DOI 10.1111/j.1432-1033.1995.tb20447.x
  • [4] Briese Bernd Holger, 1994, Journal of Environmental Polymer Degradation, V2, P75, DOI 10.1007/BF02074776
  • [5] BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF THE PSEUDOMONAS-LEMOIGNEI POLYHYDROXYALKANOATE DEPOLYMERASE SYSTEM
    JENDROSSEK, D
    FRISSE, A
    BEHRENDS, A
    ANDERMANN, M
    KRATZIN, HD
    STANISLAWSKI, T
    SCHLEGEL, HG
    [J]. JOURNAL OF BACTERIOLOGY, 1995, 177 (03) : 596 - 607
  • [6] CLONING AND CHARACTERIZATION OF THE POLY(HYDROXYALKANOIC ACID)-DEPOLYMERASE GENE LOCUS, PHAZ1, OF PSEUDOMONAS-LEMOIGNEI AND ITS GENE-PRODUCT
    JENDROSSEK, D
    MULLER, B
    SCHLEGEL, HG
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 218 (02): : 701 - 710
  • [7] CHARACTERIZATION OF THE EXTRACELLULAR POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE OF COMAMONAS SP AND OF ITS STRUCTURAL GENE
    JENDROSSEK, D
    BACKHAUS, M
    ANDERMANN, M
    [J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1995, 41 : 160 - 169
  • [8] JENDROSSEK D, 1996, UNPUB BIODEGRADATION
  • [9] Klingbeil B, 1996, FEMS MICROBIOL LETT, V142, P215
  • [10] KURUSU Y, 1994, STUD POLYM SCI, V12, P357