Recombinant non-structural 1 (NS1) protein of dengue-2 virus interacts with human STAT3β protein

被引：37

作者：

Chua, JJE

Bhuvanakantham, R

Chow, VTK

Ng, ML

机构：

[1] Natl Univ Singapore, Programme Infect Dis, Singapore 117597, Singapore

[2] Natl Univ Singapore, Fac Med, Dept Microbiol, Flavivirol Lab, Singapore 117597, Singapore

来源：

VIRUS RESEARCH | 2005年 / 112卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

dengue virus; flaviviruses; non-structural; 1; protein; human STAT3 beta protein; protein-protein interaction; yeast; 2-hybrid;

D O I：

10.1016/j.virusres.2005.03.025

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A combination of yeast two-hybrid library screening, co-immunoprecipitation and immunofluorescence microscopy demonstrated that dengue-2 virus non-structural 1 (NS 1) protein can interact with an N-terminally truncated form of human STAT3 beta (Delta N40-STAT3 beta) protein. The NS1 protein interacted with the activated STAT3 beta protein in vesicle-like structures in the cell cytoplasm. In addition, transfection of dendritic cells with plasmid expressing NS I protein also resulted in significant induction of tumor necrosis factor-alpha (TNF alpha) and interleukin-6 (IL-6). Since the STAT3 beta protein is an acute-phase response factor, its interaction with NS 1 protein may influence the pathological changes observed in dengue fever, dengue hemorrhagic fever and dengue shock syndrome. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：85 / 94

页数：10

共 41 条

[1] Characterisation of lymphocyte response and cytokine patterns in patients with Dengue fever [J].