Protocol for the induction of arthritis in C57BL/6 mice

被引：166

作者：

Inglis, Julia J. ^{[1
]}

Simelyte, Egle ^{[1
]}

McCann, Fiona E. ^{[1
]}

Criado, Gabriel ^{[1
]}

Williams, Richard O. ^{[1
]}

机构：

[1] Imperial Coll London, Kennedy Inst, Div Rheumatol, London W6 8LH, England

来源：

NATURE PROTOCOLS | 2008年 / 3卷 / 04期

关键词：

D O I：

10.1038/nprot.2008.19

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Collagen-induced arthritis is a well-validated, but strain-dependent mouse model of rheumatoid arthritis, with H-2(q) and H-2(r) strains showing the greatest degree of susceptibility. This protocol describes the induction of arthritis in the C57BL/6 strain (H-2(b)), which forms the genetic background of the majority of genetically modified strains. This protocol involves purification of type II collagen from chicken sternums, immunization of mice, clinical assessment of arthritis and analysis of T- and B-cell responses to type II collagen. Key aspects of the protocol are the need to use chicken collagen for immunization and the importance of avoiding aggressive behavior in males. The incidence of arthritis varies from 50 to 80% and is milder than the classical collagen-induced arthritis model. This procedure takes similar to 3 months to complete.

引用

页码：612 / 618

页数：7

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