Production of hexenol in a two-enzyme system: kinetic study and modelling

被引：17

作者：

Akacha, NB ^{[1
]}

Boubaker, O

Gargouri, M

机构：

[1] Natl Inst Appl Sci & Technol, Enzyme Engn Grp, Tunis, Tunisia

[2] Natl Inst Appl Sci & Technol, Dept Phys & Instrument Engn, Tunis, Tunisia

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 23-24期

关键词：

alcohol dehydrogenase; coupled enzyme kinetics; hydroperoxide lyase; simulation;

D O I：

10.1007/s10529-005-3896-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In a two-enzyme system, successive action of hydroperoxide lyase from mint and yeast alcohol-dehydrogenase catalyses the conversion of hydroperoxy linolenic acid to hexenol. Kinetic behaviour was investigated separately for each enzyme: a lumped model based on the Michaelis-Menten approach shows the fate of the reactants in the system.

引用

页码：1875 / 1878

页数：4

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