Evaluation of [18F]FHPG as PET tracer for HSVtk gene expression

被引：18

作者：

de Vries, EFJ

van Dillen, IJ

van Waarde, A

Willemsen, ATM

Vaalburg, W

Mulder, NH

Hospers, GAP

机构：

[1] Univ Groningen Hosp, PET Ctr, NL-9700 RB Groningen, Netherlands

[2] Univ Groningen Hosp, Dept Med Oncol, NL-9700 RB Groningen, Netherlands

来源：

NUCLEAR MEDICINE AND BIOLOGY | 2003年 / 30卷 / 06期

关键词：

F-18]FHPG; positron emission tomography; gene therapy; herpes simplex virus thymidine kinase; gene expression;

D O I：

10.1016/S0969-8051(03)00048-9

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

In rats, the relationship between [F-18]FHPG accumulation and HSVtk expression was studied with PET and autoradiography. [F-18]FHPG distribution closely corresponded with HSVtk immunohistochemical staining. ROI analysis of tracer uptake 2 hours p.i. and Patlak graphical analysis were applied to quantify the PET data. For both analysis methods, the [F-18]FHPG PET signal correlated well with the fraction of HSVtk expressing cells implanted, but showed a plateau when plotted against HSVtk protein levels. This might be due to rate limiting [F-18]FHPG membrane transport. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：651 / 660

页数：10

共 30 条

[1] Synthesis and preliminary evaluation of 9-(4-[18F]-fluoro-3-hydroxymethylbutyl)guanine ([18F]FHBG):: A new potential imaging agent for viral infection and gene therapy using PET [J].