A plant-specific dynamin-related protein forms a ring at the chloroplast division site

被引:150
作者
Miyagishima, S [1 ]
Nishida, K
Mori, T
Matsuzaki, M
Higashiyama, T
Kuroiwa, H
Kuroiwa, T
机构
[1] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan
[2] Univ Tokyo, Grad Sch Med, Dept Biomed Chem, Tokyo 1130033, Japan
[3] Biooriented Technol Res Advancement Inst, Tokyo 1050001, Japan
关键词
D O I
10.1105/tpc.009373
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chloroplasts have retained the bacterial FtsZ for division, whereas mitochondria lack FtsZ except in some lower eukaryotes. Instead, mitochondrial division involves a dynamin-related protein, suggesting that chloroplasts retained the bacterial division system, whereas a dynamin-based system replaced the bacterial system in mitochondria during evolution. In this study, we identified a novel plant-specific group of dynamins from the primitive red alga Cyanidioschyzon merolae. Synchronization of chloroplast division and immunoblot analyses showed that the protein (CmDnm2) associates with the chloroplast only during division. Immunocytochemical analyses showed that CmDnm2 appears in cytoplasmic patches just before chloroplast division and is recruited to the cytosolic side of the chloroplast division site to form a ring in the late stage of division. The ring constricts until division is complete, after which it disappears. These results show that a dynamin-related protein also participates in chloroplast division and that its behavior differs from that of FtsZ and plastid-dividing rings that form before constriction at the site of division. Combined with the results of a recent study of mitochondrial division in Cyanidioschyzon, our findings led us to hypothesize that when first established in lower eukaryotes, mitochondria and chloroplasts divided using a very similar system that included the FtsZ ring, the plastid-dividing/mitochondrion-dividing ring, and the dynamin ring.
引用
收藏
页码:655 / 665
页数:11
相关论文
共 57 条
  • [1] STUDIES WITH CYANIDIUM CALDARIUM, AN ANOMALOUSLY PIGMENTED CHLOROPHYTE
    ALLEN, MB
    [J]. ARCHIV FUR MIKROBIOLOGIE, 1959, 32 (03): : 270 - 277
  • [2] A dynamin-like protein (ADL2b), rather than FtsZ, is involved in Arabidopsis mitochondrial division
    Arimura, S
    Tsutsumi, N
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) : 5727 - 5731
  • [3] Mitochondrial FtsZ in a chromophyte alga
    Beech, PL
    Nheu, T
    Schultz, T
    Herbert, S
    Lithgow, T
    Gilson, PR
    McFadden, GI
    [J]. SCIENCE, 2000, 287 (5456) : 1276 - 1279
  • [4] FTSZ RING STRUCTURE ASSOCIATED WITH DIVISION IN ESCHERICHIA-COLI
    BI, E
    LUTKENHAUS, J
    [J]. NATURE, 1991, 354 (6349) : 161 - 164
  • [5] The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast
    Bleazard, W
    McCaffery, JM
    King, EJ
    Bale, S
    Mozdy, A
    Tieu, Q
    Nunnari, J
    Shaw, JM
    [J]. NATURE CELL BIOLOGY, 1999, 1 (05) : 298 - 304
  • [6] Bacterial cell division
    Bramhill, D
    [J]. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1997, 13 : 395 - 424
  • [7] Division of mitochondria requires a novel DNM1-interacting protein, net2p
    Cerveny, KL
    McCaffery, JM
    Jensen, RE
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (02) : 309 - 321
  • [8] Gag3p, an outer membrane protein required for fission of mitochondrial tubules
    Fekkes, P
    Shepard, KA
    Yaffe, MP
    [J]. JOURNAL OF CELL BIOLOGY, 2000, 151 (02) : 333 - 340
  • [9] The GTPase effector domain sequence of the Dnm1p GTPase regulates self-assembly and controls a rate-limiting step in mitochondrial fission
    Fukushima, NH
    Brisch, E
    Keegan, BR
    Bleazard, W
    Shaw, JM
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (09) : 2756 - 2766
  • [10] Cell division protein FtsZ: running rings around bacteria, chloroplasts and mitochondria
    Gilson, PR
    Beech, PL
    [J]. RESEARCH IN MICROBIOLOGY, 2001, 152 (01) : 3 - 10