Real-Time SNP Analysis in Secondary-Structure-Folded Nucleic Acids

被引：47

作者：

Grimes, Jeffrey ^{[1
]}

Gerasimova, Yulia V. ^{[1
]}

Kolpashchikov, Dmitry M. ^{[1
]}

机构：

[1] Univ Cent Florida, Dept Chem, Orlando, FL 32816 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 47期

关键词：

biotransformations; genotyping; molecular beacons; real-time detection; tau proteins; SINGLE NUCLEOTIDE POLYMORPHISMS; MOLECULAR BEACONS; PNA HYBRIDIZATION; ENERGY-TRANSFER; HUMAN GENOME; DNA; PROBES; SEQUENCES; RNA; DEOXYRIBOZYME;

D O I：

10.1002/anie.201004475

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A tricomponent sensor was designed for genotyping stem-loop-structured DNA sequences. DNA adaptor strand f (see picture; red) hybridizes to the analyte (orange) with high affinity and unwinds its secondary structure; strand m (green) forms a stable complex only with the fully complementary analyte sequence. A fluorescing molecular beacon reports complex formation. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：8950 / 8953

页数：4

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