Linear polyethylenimine as a tool for comparative studies of antisense and short double-stranded RNA oligonucleotides

被引：19

作者：

Bologna, JC ^{[1
]}

Dorn, G ^{[1
]}

Natt, F ^{[1
]}

Weiler, J ^{[1
]}

机构：

[1] Novartis Pharma AG, Funct Genom, CH-4002 Basel, Switzerland

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2003年 / 22卷 / 5-8期

关键词：

D O I：

10.1081/NCN-120023124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite the recently enlarged field of available RNA knock-down technologies, e.g., antisense oligonucleotides (ASOs) and duplexes of synthetic 21 nucleotides RNAs (siRNAs), no versatile transfection reagent has been reported to deliver different nucleic acids formats at high rates of efficiency. We have evaluated the versatility and efficacy of linear PEI in transfecting and properly delivering a broad panel of nucleic acids such as short oligonucleotides and double-stranded RNA into cells in culture.

引用

页码：1729 / 1731

页数：3