Differential proteomics for studying Drosophila immunity

被引：10

作者：

Vierstraete, E ^{[1
]}

Verleyen, P ^{[1
]}

De Loof, A ^{[1
]}

Schoofs, L ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Lab Dev Physiol Genom & Prot, B-3000 Louvain, Belgium

来源：

TRENDS IN COMPARATIVE ENDOCRINOLOGY AND NEUROBIOLOGY | 2005年 / 1040卷

关键词：

two-dimensional difference gel electrophoresis; secretome; immune response proteins; Drosophila melanogaster;

D O I：

10.1196/annals.1327.104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we report the identification of proteins associated with the immune response of Drosophila by analyzing the hemolymph profiles after infection. Two-dimensional difference gel electrophoresis was used to study the secretome in the hemolymph of Drosophila larvae. Shortly after induction with lipopolysaccharides, we identified 10 proteins, which we designated "Drosophila instantly released immune proteins". Infection with Micrococcus luteus or Saccharomyces cerevisiae induced 20 and 19 differential protein spots, respectively. Next to known immune proteins, new candidates that require further investigation were identified.

引用

页码：504 / 507

页数：4

共 6 条

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[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 304 (04) : 831 - 838

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