Conformational changes of photoactive yellow protein monitored by terahertz spectroscopy

被引：59

作者：

Castro-Camus, E. ^{[1
]}

Johnston, M. B. ^{[1
]}

机构：

[1] Univ Oxford, Dept Phys, Clarendon Lab, Oxford OX1 3PU, England

来源：

CHEMICAL PHYSICS LETTERS | 2008年 / 455卷 / 4-6期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1016/j.cplett.2008.02.084

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Observing the structural dynamics of proteins under conditions as close as possible to those in a living organism is essential for understanding the biological functions of proteins accurately. Here we demonstrate that terahertz spectroscopy is a convenient probe of conformational changes in proteins suspended in physiological buffer solution. We have observed that the partial unfolding of photoactive yellow protein leads to a clear increase in absorption at terahertz frequencies. Using normal mode and molecular dynamics simulations we show that this increase in absorption is related to an increase in the density of delocalised vibrational modes in the more flexible partially unfolded state. (c) 2008 Elsevier B. V. All rights reserved.

引用

页码：289 / 292

页数：4

共 31 条

[1] Molecular dynamics simulations of photoactive yellow protein (PYP) in three states of its photocycle: a comparison with X-ray and NMR data and analysis of the effects of Glu46 deprotonation and mutation [J].

Antes, I ;

Thiel, W ;

van Gunsteren, WF .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2002, 31 (07) :504-520

[2] The solution structure of a transient photoreceptor intermediate:: Δ25 photoactive yellow protein [J].

Bernard, C ;

Houben, K ;

Derix, NM ;

Marks, D ;

van der Horst, MA ;

Hellingwerf, KJ ;

Boelens, R ;

Kaptein, R ;

van Nuland, NAJ .

STRUCTURE, 2005, 13 (07) :953-962

[3] NORMAL-MODES FOR SPECIFIC MOTIONS OF MACROMOLECULES - APPLICATION TO THE HINGE-BENDING MODE OF LYSOZYME [J].

BROOKS, B ;

KARPLUS, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (15) :4995-4999

[4] HARMONIC DYNAMICS OF PROTEINS - NORMAL-MODES AND FLUCTUATIONS IN BOVINE PANCREATIC TRYPSIN-INHIBITOR [J].

BROOKS, B ;

KARPLUS, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (21) :6571-6575

[5] Large oxidation dependence observed in terahertz dielectric response for cytochrome c [J].

Chen, JY ;

Knab, JR ;

Cerne, J ;

Markelz, AG .

PHYSICAL REVIEW E, 2005, 72 (04)

[6] Nonlinear infrared spectroscopy of protein conformational change during thermal unfolding [J].

Chung, HS ;

Khalil, M ;

Tokmakoff, A .

JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (39) :15332-15342

[7] Structure at 0.85 Å resolution of an early protein photocycle intermediate [J].

Genick, UK ;

Soltis, SM ;

Kuhn, P ;

Canestrelli, IL ;

Getzoff, ED .

NATURE, 1998, 392 (6672) :206-209

[8] Anticipatory active-site motions and chromophore distortion prime photoreceptor PYP for light activation [J].

Getzoff, ED ;

Gutwin, KN ;

Genick, UK .

NATURE STRUCTURAL BIOLOGY, 2003, 10 (08) :663-668

[9] Photoactive Yellow Protein, a new type of photoreceptor protein: Will this "yellow lab" bring us where we want to go? [J].

Hellingwerf, KJ ;

Hendriks, J ;

Gensch, T .

JOURNAL OF PHYSICAL CHEMISTRY A, 2003, 107 (08) :1082-1094

[10] Solute-induced retardation of water dynamics probed directly by terahertz spectroscopy [J].

Heugen, U. ;

Schwaab, G. ;

Bruendermann, E. ;

Heyden, M. ;

Yu, X. ;

Leitner, D. M. ;

Havenith, M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (33) :12301-12306

← 1 2 3 4 →