Nucleocapsid-like structures of Ebola virus reconstructed using electron tomography

被引：30

作者：

Noda, T

Aoyama, K

Sagara, H

Kida, H

Kawaoka, Y ^{[1
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Div Virol, Dept Microbiol & Immunol,Minato Ku, Tokyo 1088639, Japan

[2] Univ Tokyo, Inst Med Sci, Fine Morphol Lab, Dept Basic Med Sci, Tokyo 1088639, Japan

[3] Hokkaido Univ, Microbiol Lab, Dept Dis Contol, Grad Sch Vet Med, Sapporo, Hokkaido 0600818, Japan

[4] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2005年 / 67卷 / 03期

关键词：

Ebola virus; electron tomography; nucleocapsid;

D O I：

10.1292/jvms.67.325

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Electron tomography (ET) is a new technique for high resolution, three-dimensional (3D) reconstruction of pleiomorphic macromolecular complexes, such as virus components. By employing this technique, we resolved the 3D structure of Ebola virus nucleocapsid-like (NC-like) structures in the cytoplasm of cells expressing NP, VP24, and VP35: the minimum components required to form these NC-like structures. Reconstruction of these tubular NC-like structures of Ebola virus showed them to be composed of left-handed helices spaced at short intervals, which is structurally consistent with other non-segmented negative-strand RNA viruses.

引用

页码：325 / 328

页数：4

共 14 条

[1] Electron tomography: towards visualizing the molecular organization of the cytoplasm [J].