Sedimentation equilibrium: a valuable tool to study homologous and heterogeneous interactions of proteins or proteins and nucleic acids

被引：6

作者：

Behlke, J ^{[1
]}

Ristau, O ^{[1
]}

机构：

[1] Max Delbruck Ctr Mol Med, D-13092 Berlin, Germany

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2003年 / 32卷 / 05期

关键词：

analytical ultracentrifugation; binding constants; heterogeneous association; self-association; virial coefficients;

D O I：

10.1007/s00249-003-0318-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We present a short overview of our experience in analyzing the affinity and stoichiometry of self-associating and heterologous interactions by using the sedimentation equilibrium technique. Data acquisition and the fitting procedure employing the computer programs that we have developed, Polymole and Virial, are utilized for obtaining reliable results under ideal as well as non-ideal conditions. Such data derived from biologically important macromolecules find utility in understanding physiological events such as cell regulation.

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页码：427 / 431

页数：5

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